Prahalad Agasanur K, Hickey Robert J, Huang Jeffrey, Hoelz Derek J, Dobrolecki Lacey, Murthy Sreemala, Winata Therry, Hock Janet M
Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, 46202, USA.
Proteomics. 2006 Jun;6(12):3482-93. doi: 10.1002/pmic.200500929.
Parathyroid hormone (amino acids 1-34) (PTH) regulates bone and calcium homeostasis. The magnitude of the effects of PTH on bone varies in osteoporosis patients. We employed ProteinChip technology to generate protein profiles from sera of mice treated once daily with PTH or vehicle for 3 or 11 days. Data analyses on selected arrays indicated significant increases in serum proteins or peptides in PTH-treated groups, compared to vehicle-controls. The magnitude of change increased with duration of treatment. Anion-exchange fractionation of sera prior to profiling on array surfaces increased the number of proteins detected that were regulated by PTH. The optimized purification conditions developed "on-chip" for subsets of proteins, reflected corresponding behavior with process-compatible chromatographic resins under elution chromatography. We have identified and evaluated subsets of serum proteins regulated by PTH treatment, using a combination of ProteinChip technology, column chromatography, PAGE and LC-MS/MS. Our data demonstrate the feasibility of using a panel of serum proteins to detect PTH responsiveness in humans.
甲状旁腺激素（氨基酸1 - 34）（PTH）调节骨骼和钙稳态。PTH对骨骼的影响程度在骨质疏松症患者中有所不同。我们采用蛋白质芯片技术，从每天接受一次PTH或赋形剂处理3天或11天的小鼠血清中生成蛋白质谱。对选定阵列的数据分析表明，与赋形剂对照组相比，PTH处理组的血清蛋白质或肽显著增加。变化幅度随治疗持续时间增加。在阵列表面进行分析之前，对血清进行阴离子交换分级分离增加了检测到的受PTH调节的蛋白质数量。为蛋白质亚群在“芯片上”开发的优化纯化条件，反映了在洗脱色谱下与过程兼容的色谱树脂的相应行为。我们使用蛋白质芯片技术、柱色谱、PAGE和LC-MS/MS相结合的方法，鉴定并评估了受PTH处理调节的血清蛋白质亚群。我们的数据证明了使用一组血清蛋白质来检测人类对PTH反应性的可行性。
