Celik Ayla
Department of Biology, Faculty of Science and Letters, Mersin University, Mersin, Turkey.
Drug Chem Toxicol. 2006;29(2):227-36. doi: 10.1080/01480540600566832.
The genotoxic effect of CBZ has been investigated in few studies. There is little evidence linking carbamazepine (CBZ) with any genotoxic effects, particularly in vitro micronucleus test using cytogenesis-block technique. In this study, the genotoxicity of the antiepileptic drug, carbamazepine, was tested using cytokinesis-block (CB) micronucleus assay. In vitro analysis was performed in human blood lymphocytes from four healthy persons at five different concentrations of carbamazepine (6, 8, 10, 12, 14 microg/mL). Genotoxic potential and cytotoxic effects of carbamazepine were evaluated by using micronucleus assay and cytokinesis-block proliferation index (CBPI), called the parameter of cytotoxicity in human peripheral blood lymphocyte cultures, respectively. The results of this study indicate that CBZ caused the genotoxic effect under in vitro conditions, except at the dose of 6 microg/mL, and cytotoxic effects of carbamazepine were revealed by a decrease in the cytokinesis-block proliferation index at all the concentrations.
关于卡马西平（CBZ）的遗传毒性作用，仅有少量研究进行过探究。几乎没有证据表明卡马西平（CBZ）具有任何遗传毒性作用，尤其是在采用细胞分裂阻断技术的体外微核试验中。在本研究中，使用细胞分裂阻断（CB）微核试验检测了抗癫痫药物卡马西平的遗传毒性。对来自四名健康人的人血淋巴细胞进行体外分析，卡马西平设置了五个不同浓度（6、8、10、12、14微克/毫升）。分别通过微核试验和细胞分裂阻断增殖指数（CBPI，这是人类外周血淋巴细胞培养中细胞毒性的参数）评估了卡马西平的遗传毒性潜力和细胞毒性作用。本研究结果表明，除了6微克/毫升剂量外，卡马西平在体外条件下会产生遗传毒性作用，并且在所有浓度下，细胞分裂阻断增殖指数的降低都表明了卡马西平的细胞毒性作用。
