Li Weihua, Tsubouchi Ryoko, Qiao Shanlou, Haneda Miyako, Murakami Keiko, Yoshino Masataka
Department of Biochemistry, Aichi Medical University School of Medicine, Aichi, Japan.
Biomed Res. 2006 Apr;27(2):69-74. doi: 10.2220/biomedres.27.69.
Effects of eugenol compounds on the production of nitric oxide (NO) in RAW264.7 macrophages were analyzed in relation to the anti-inflammatory action of these compounds. Eugenol and isoeugenol inhibited lipopolysaccharide (LPS)-dependent production of NO, which was due to the inhibition of protein synthesis of inducible nitric oxide synthase (iNOS). Isoeugenol showed the most effective inhibitory effect and eugenol was less effective. LPS-dependent expression of cyclooxygenase-2 (COX-2) protein was also inhibited markedly by isoeugenol, and less effectively by eugenol. Anti-inflammatory action of eugenol compounds may be explained by the inhibition of NO production and COX-2 expression, the pro-inflammatory mediators.
分析了丁香酚化合物对RAW264.7巨噬细胞中一氧化氮（NO）产生的影响，并探讨了这些化合物的抗炎作用。丁香酚和异丁香酚抑制脂多糖（LPS）依赖性NO的产生，这是由于抑制了诱导型一氧化氮合酶（iNOS）的蛋白质合成。异丁香酚显示出最有效的抑制作用，而丁香酚的效果较差。异丁香酚还显著抑制了LPS依赖性环氧化酶-2（COX-2）蛋白的表达，丁香酚的抑制作用较弱。丁香酚化合物的抗炎作用可能是通过抑制NO产生和COX-2表达（促炎介质）来解释的。
