原位导管灌注法在促进从小鼠胰腺中分离高质量RNA方面的应用。

Application of in situ ductal perfusion to facilitate isolation of high-quality RNA from mouse pancreas.

作者信息

Mullin Anne E, Soukatcheva Galina, Verchere C Bruce, Chantler Janet K

机构信息

University of British Columbia, Vancouver and Child and Family Research Institute, Vancouver, BC, Canada.

出版信息

Biotechniques. 2006 May;40(5):617-21. doi: 10.2144/000112146.

DOI:10.2144/000112146

PMID:16708761

Abstract

A technique to isolate high-quality intact RNA from murine pancreas is described. This technique involves in situ ductal perfusion of the pancreas with an RNase inhibitor prior to removal of the organ for RNA extraction. In this way, the pancreatic RNases are inhibited in situ allowing good yields of intact RNA, suitable for studies on pancreatic gene transcription by real-time PCR or microarray analysis, to be obtained in a reliable way.

摘要

本文描述了一种从小鼠胰腺中分离高质量完整RNA的技术。该技术包括在摘除胰腺进行RNA提取之前，用核糖核酸酶抑制剂对胰腺进行原位导管灌注。通过这种方式，胰腺核糖核酸酶在原位被抑制，从而能够以可靠的方式获得大量完整的RNA，适用于通过实时PCR或微阵列分析进行胰腺基因转录研究。