Paixão Valéria A, Vidal Daniel O, Caballero Otavia L, Vettore André L, Tone Luiz G, Ribeiro Karina Braga, Lopes Luiz Fernando
Ludwig Institute for Cancer Research, São Paulo Branch, São Paulo, SP, Brazil.
Leuk Res. 2006 Jul;30(7):891-4. doi: 10.1016/j.leukres.2005.11.016. Epub 2006 May 18.
Promoter hypermethylation occurs early in leukemogenesis and seems to be associated with poor prognosis in acute lymphoblastic leukemia (ALL). The methylation status of the promoter region of six genes was analyzed in 71 children with ALL using methylation specific PCR (MSP). Calcitonin (CALCA) and E-cadherin (CDH1) were the most frequently methylated genes in this group of patients. Considering the patients with central nervous system (CNS) infiltration, the estimated 2-year overall survival (OS) was 20% for those with methylation in CALCA promoter and 85% for those without (p=0.001). Our results suggest that the hypermethylation of CALCA promoter is a promising prognostic marker and may predict a higher risk for ALL patients with CNS infiltration.
启动子高甲基化在白血病发生早期出现，且似乎与急性淋巴细胞白血病（ALL）的不良预后相关。使用甲基化特异性PCR（MSP）分析了71例ALL患儿6个基因启动子区域的甲基化状态。降钙素（CALCA）和E-钙黏蛋白（CDH1）是该组患者中最常发生甲基化的基因。考虑有中枢神经系统（CNS）浸润的患者，CALCA启动子甲基化者的估计2年总生存率（OS）为20%，未甲基化者为85%（p=0.001）。我们的结果表明，CALCA启动子的高甲基化是一个有前景的预后标志物，可能预示ALL合并CNS浸润患者的更高风险。
