Khazaei M, Nematbakhsh M
Department of Physiology and Pharmacology, Medical University of Birjand, Birjand, Iran.
Regul Pept. 2006 Jul 15;135(1-2):91-4. doi: 10.1016/j.regpep.2006.04.003. Epub 2006 May 19.
CardioVascular Disease (CVD) accounts for considerable mortality and morbidity in developed countries. Most of the common forms of CVD, such as hypertension, are caused by functional and structural changes in endothelial function. This study was designed to study the effect of hypertension on serum Nitric Oxide (NO) and Vascular Endothelial Growth Factor (VEGF) concentrations in DOCA-Salt hypertensive ovariectomized rats. Thirty female rats were ovariectomized. Blood samples were taken and the animals were divided into hypertensive and control groups. Hypertension was induced by DOCA-Salt method. DOCA was injected 30 mg/kg of body weight subcutaneously, twice a week with NaCl 1% instead of tap water for drinking throughout the experiment. The control group received normal saline injection with usual drinking water. Results showed that serum NO concentration in DOCA-Salt hypertensive rats was lower than the control group (18.35 +/- 5.31, 45.01 +/- 12.54 micromol/l, respectively) (p < 0.05). Also, the mean serum VEGF concentration was raised after induced hypertension (120.55 +/- 8.11 vs. 88.58 +/- 2.24 pg/ml) (p < 0.05). In conclusion, reduced serum NO and increased serum VEGF concentrations in hypertensive animals support the concept of endothelial dysfunction in hypertensive subjects.
心血管疾病（CVD）在发达国家导致了相当高的死亡率和发病率。大多数常见形式的心血管疾病，如高血压，是由内皮功能的功能和结构变化引起的。本研究旨在研究高血压对去氧皮质酮盐（DOCA）-盐诱导的高血压去卵巢大鼠血清一氧化氮（NO）和血管内皮生长因子（VEGF）浓度的影响。30只雌性大鼠接受了卵巢切除术。采集血样后，将动物分为高血压组和对照组。采用DOCA-盐法诱导高血压。皮下注射DOCA，剂量为30mg/kg体重，每周两次，整个实验期间饮用1%的氯化钠溶液代替自来水。对照组注射生理盐水并饮用普通饮用水。结果显示，DOCA-盐高血压大鼠的血清NO浓度低于对照组（分别为18.35±5.31、45.01±12.54μmol/L）（p<0.05）。此外，诱导高血压后血清VEGF平均浓度升高（120.55±8.11对88.58±2.24pg/ml）（p<0.05）。总之，高血压动物血清NO降低和血清VEGF升高支持高血压患者存在内皮功能障碍的观点。
