Chen Zhijian, Lou Jianlin, Chen Shijie, Zheng Wei, Wu Wei, Jin Lifen, Deng Hongping, He Jiliang
Zhejiang University, Medical College, Institute of Occupational and Environmental Health, Hangzhou 310006, Zhejiang, People's Republic of China.
Toxicology. 2006 Jun 15;223(3):219-26. doi: 10.1016/j.tox.2006.03.016. Epub 2006 Apr 1.
To evaluate the genotoxic effects of lead (Pb) exposure, 25 workers in a workplace producing storage battery were monitored for three genetic end-points using micronucleus (MN) assay, comet assay and TCR gene mutation test. Twenty-five controls were matched with workers according to age, gender and smoking. The air Pb concentration in the workplace was 1.26 mg/m(3). All subjects were measured for Pb concentration of blood by atom absorption spectrophotometry. The mean Pb concentration of blood in workers (0.32 mg/l) was significantly higher than that in controls (0.02 mg/l). The results of MN test showed that the mean micronuclei rate (MNR) and mean micronucleated cells rate (MCR) in workers were 9.04+/-1.51 per thousand and 7.76+/-1.23 per thousand, respectively, which were significantly higher than those (2.36+/-0.42 per thousand and 1.92+/-0.31 per thousand) in controls (P<0.01). It was found in the comet assay that the mean tail length (MTL) of 25 workers and 25 controls were 2.42+/-0.09 and 1.02+/-0.08 microm, respectively, there was significant difference between workers and controls for MTL (P<0.01), also the difference of the mean tail moment (MTM) between workers (0.85+/-0.05) and controls (0.30+/-0.09) was very significant (P<0.01). However, in TCR gene mutation assay Mfs-TCR of workers and controls were 1.69+/-0.15 x 10(-4) and 1.74+/-0.17 x 10(-4), respectively, there was no significant difference between workers and controls (P>0.05). The results of our study indicated that the genetic damage was detectable in 25 workers occupationally exposed to lead.
为评估铅（Pb）暴露的遗传毒性效应，对一家生产蓄电池工作场所的25名工人，采用微核（MN）试验、彗星试验和TCR基因突变试验监测三项遗传终点。根据年龄、性别和吸烟情况，为25名工人匹配了对照。工作场所空气中铅浓度为1.26 mg/m³。采用原子吸收分光光度法测量所有受试者的血铅浓度。工人的平均血铅浓度（0.32 mg/l）显著高于对照组（0.02 mg/l）。微核试验结果显示，工人的平均微核率（MNR）和平均微核细胞率（MCR）分别为9.04±1.51‰和7.76±1.23‰，显著高于对照组（2.36±0.42‰和1.92±0.31‰）（P<0.01）。彗星试验发现，25名工人和25名对照的平均尾长（MTL）分别为2.42±0.09和1.02±0.08微米，工人与对照之间的MTL存在显著差异（P<0.01），工人（0.85±0.05）与对照（0.30±0.09）之间的平均尾矩（MTM）差异也非常显著（P<0.01）。然而，在TCR基因突变试验中，工人和对照的Mfs-TCR分别为1.69±0.15×10⁻⁴和1.74±0.17×10⁻⁴，工人与对照之间无显著差异（P>0.05）。我们的研究结果表明，在25名职业性铅暴露工人中可检测到遗传损伤。
