Kondo Akira, Hirakata Yoichi, Gotoh Naomasa, Fukushima Kazuko, Yanagihara Katsunori, Ohno Hideaki, Higashiyama Yasuhito, Miyazaki Yoshitugu, Nishide Kiyoharu, Node Manabu, Yamada Yasuaki, Kohno Shigeru, Kamihira Shimeru
Department of Laboratory Medicine, Nagasaki University School of Medicine and Dentistry, Nagasaki, Nagasaki 852-850, Japan.
Microbiol Immunol. 2006;50(5):395-401. doi: 10.1111/j.1348-0421.2006.tb03806.x.
We studied the quorum sensing (QS) system and the related homoserine lactones (HSLs) observing Pseudomonas aeruginosa invasion using the epithelial cell monolayer penetration assay model. Compared to the PAO1 wild-type, the QS mutants, DeltalasI and DeltarhlI, were compromised in their capacity to invade. The decreased invasiveness of DeltarhlI was restored by adding 100 microM exogenous C(4)-HSL. However, the decreased invasiveness of an efflux mutant, DeltamexAB-oprM, was not restored in the presence of exogenous HSLs. The QS system partially plays a role in P. aeruginosa invasion; however, C(4)-HSL and 3-O-C(12)-HSL are not the essential determinants for invasiveness for P. aeruginosa.
我们使用上皮细胞单层穿透试验模型研究了群体感应（QS）系统以及相关的高丝氨酸内酯（HSLs），观察铜绿假单胞菌的侵袭情况。与PAO1野生型相比，QS突变体DeltalasI和DeltarhlI的侵袭能力受损。通过添加100 microM外源性C(4)-HSL可恢复DeltarhlI降低的侵袭性。然而，在外源性HSLs存在的情况下，外排突变体DeltamexAB-oprM降低的侵袭性并未恢复。QS系统在铜绿假单胞菌的侵袭中部分发挥作用；然而，C(4)-HSL和3-O-C(12)-HSL并非铜绿假单胞菌侵袭性的必需决定因素。
