Yamaguchi Akira, Nakano Masaki, Nochi Kimihisa, Yamashita Tomohisa, Morita Kotaro, Teramae Norio
Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai, 980-8578, Japan.
Anal Bioanal Chem. 2006 Oct;386(3):627-32. doi: 10.1007/s00216-006-0470-x. Epub 2006 May 20.
The adsorption and longitudinal diffusion behaviors of a series of hemicyanine dyes to phospholipid vesicle membranes were studied by second-harmonic generation (SHG) and fluorescence spectroscopies. It was observed that the longitudinal diffusion of cationic hemicyanine dyes takes place immediately after the initial adsorption of these dyes to the outer surface of the vesicle membrane. In contrast, hardly any amount of a zwitterionic hemicyanine dye with a sulfonate group diffused across the vesicle membrane within the measurement time (<2000 s). Based on the difference in the time-course responses of SHG and fluorescence spectroscopies for all of the hemicyanine dyes tested, we propose that hydration of the sulfonate group is mainly responsible for the low diffusivity of the zwitterionic hemicyanine dye.
通过二次谐波产生（SHG）和荧光光谱法研究了一系列半菁染料对磷脂囊泡膜的吸附和纵向扩散行为。观察到阳离子半菁染料在最初吸附到囊泡膜外表面后立即发生纵向扩散。相比之下，在测量时间（<2000秒）内，几乎没有磺酸根基团的两性离子半菁染料扩散穿过囊泡膜。基于所有测试的半菁染料的SHG和荧光光谱时间进程响应的差异，我们提出磺酸根基团的水合作用是两性离子半菁染料低扩散率的主要原因。
