Stern Norman J, Pretanik Stephen
U.S. Department of Agriculture, Agricultural Research Service, South Atlanta Area, Poultry Microbiological Safety Research Unit, Russell Research Center, Athens, Georgia 30604, USA.
J Food Prot. 2006 May;69(5):1034-9. doi: 10.4315/0362-028x-69.5.1034.
Foodborne Campylobacter-associated gastroenteritis remains a public health concern, and the Centers for Disease Control and Prevention suggests that improperly handled poultry is the most important source of this human disease. In response to these concerns, 10 of the largest U.S. poultry integrators cooperatively determined the incidence and counts of Campylobacter on processed broiler carcasses. Prior to conducting the survey, laboratory personnel were trained in a direct Campy-Cefex plating procedure for enumeration of the organism. Before and after the survey enumeration, consistency in reporting was compared among the participating laboratories. Participating laboratories were able to consistently estimate inoculated concentrations of Campylobacter in carcass rinses. Within the central study, we determined the potential exposure of U.S. consumers to Campylobacter spp. associated with broiler carcasses during a 13-month period. Among each of the 13 participating poultry complexes, rinses from 25 randomly selected fully processed carcasses were sampled monthly from individual flocks. Among 4200 samples, approximately 74% of the carcasses yielded no countable Campylobacter cells. Campylobacter spp. were isolated from approximately 3.6% of all commercially processed broiler carcasses at more than 10(5) CFU per carcass. Acceptable counts of these organisms on raw poultry carcasses remain to be determined. Nevertheless, this survey indicates industry recognition of its responsibility to assess and reduce public exposure to Campylobacter through broiler chickens.
食源性弯曲杆菌相关的肠胃炎仍是一个公共卫生问题，疾病控制与预防中心指出，家禽处理不当是这种人类疾病最重要的来源。针对这些担忧，美国10家最大的家禽综合企业合作确定了加工后的肉鸡胴体上弯曲杆菌的发生率和数量。在开展调查之前，实验室人员接受了直接使用弯曲杆菌-头孢克肟平板计数法对该微生物进行计数的培训。在调查计数前后，对参与调查的实验室之间报告的一致性进行了比较。参与调查的实验室能够一致地估计胴体冲洗液中接种的弯曲杆菌浓度。在核心研究中，我们确定了美国消费者在13个月期间接触与肉鸡胴体相关的弯曲杆菌属的潜在情况。在13个参与调查的家禽养殖场中，每月从各个鸡群中随机选取25只完全加工好的胴体进行冲洗取样。在4200个样本中，约74%的胴体未检出可计数的弯曲杆菌细胞。弯曲杆菌属在所有商业加工的肉鸡胴体中约3.6%的胴体上被分离出来，每具胴体的菌落形成单位超过10⁵。生禽胴体上这些微生物的可接受数量仍有待确定。尽管如此，这项调查表明行业认识到其有责任评估并减少公众通过肉鸡接触弯曲杆菌的情况。
