Tsuruta Shunsuke, Matsumoto Mishiya, Fukuda Shiro, Yamashita Atsuo, Cui Ying Jun, Wakamatsu Hiroya, Sakabe Takefumi
Department of Anesthesiology-Resuscitology, Yamaguchi University School of Medicine, Yamaguchi, Japan.
Anesth Analg. 2006 Jun;102(6):1722-7. doi: 10.1213/01.ane.0000216006.82190.4a.
We examined the effects of cyclosporin A (CsA), a drug that inhibits mitochondrial permeability transition pore, and insulin on ischemic spinal cord damage in rabbits. We assigned rabbits to 5 groups (n = 6 in each); sham barrier-opened group (sham BO), barrier-opened group (BO), barrier-opened-CsA group (BO-CsA), barrier-opened-insulin group (BO-I), and barrier-opened-CsA-insulin group (BO-CsA-I). The blood-spinal cord barrier was opened to facilitate drug penetration by a mild injury to the lumber spinal cord on day 1. CsA (10 mg/kg per day IV) was administered on day 3 to day 5 (total 30 mg/kg). Insulin was administered 30 min before ischemia. In all groups, spinal cord ischemia was produced on day 5 by occluding the abdominal aorta for 13 min. Neurological and histopathological evaluations were performed 4 days after ischemia. In group BO-CsA, blood glucose concentrations were significantly larger compared with the other four groups, and no protection was observed. In contrast, hindlimb motor function in groups BO-I and Bo-CsA-I and histopathology in group BO-CsA-I were significantly better than in groups sham BO, BO, and BO-CsA. The results indicate that insulin protects against ischemic spinal cord injury, whereas the effect of CsA is, at best, minimal.
我们研究了环孢素A（CsA，一种抑制线粒体通透性转换孔的药物）和胰岛素对兔缺血性脊髓损伤的影响。我们将兔分为5组（每组n = 6）；假手术屏障开放组（假手术BO）、屏障开放组（BO）、屏障开放 - CsA组（BO - CsA）、屏障开放 - 胰岛素组（BO - I）和屏障开放 - CsA - 胰岛素组（BO - CsA - I）。在第1天，通过对腰段脊髓进行轻度损伤来开放血脊髓屏障，以促进药物渗透。在第3天至第5天给予CsA（每天静脉注射10 mg/kg，共30 mg/kg）。在缺血前30分钟给予胰岛素。在所有组中，于第5天通过阻断腹主动脉13分钟造成脊髓缺血。在缺血4天后进行神经学和组织病理学评估。在BO - CsA组中，血糖浓度与其他四组相比显著更高，且未观察到保护作用。相比之下，BO - I组和BO - CsA - I组的后肢运动功能以及BO - CsA - I组的组织病理学明显优于假手术BO组、BO组和BO - CsA组。结果表明，胰岛素可保护免受缺血性脊髓损伤，而CsA的作用充其量是最小的。
