Bonen Arend, Nickerson James G, Momken Iman, Chabowski Adrian, Calles-Escandon Jorge, Tandon Narendra N, Glatz Jan F C, Luiken Joost J F P
Department of Human Health and Nutritional Sciences University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
Mol Cell Biochem. 2006 Oct;291(1-2):145-54. doi: 10.1007/s11010-006-9208-5. Epub 2006 May 23.
Rates of fatty acid oxidation increase rapidly in both rat heart and skeletal muscle in the early postnatal period. Therefore, we examined in heart and soleus muscle, (a) whether there were rapid changes in fatty acid transporter (FAT/CD36, FABPpm) mRNA and protein expression early in life (days 10 -36) and thereafter (days 84, 160, 365), and (b) whether the rates of fatty acid transport and the plasmalemmal content of FAT/CD36 and FABPpm were altered. Protein expression was altered rapidly from day 10-36 in both heart (FAT/CD36 only, +21%, P < 0.05)) and soleus muscle (FAT/CD36 + 100%, P < 0.05; FABPpm -20%, P < 0.05), with no further changes thereafter (P < 0.05). Rates of fatty acid transport (day 10 vs day 160) were increased in heart (+33%, P < 0.05) and muscle (+85%, P < 0.05), and were associated with concomitant increases in plasmalemmal FABPpm (+44%, P < 0.05) and FAT/CD36 (+16%, P < 0.05) in the heart, and only plasmalemmal FAT/CD36 in muscle (+90%, P < 0.05). Therefore, known changes in the rates of fatty acid oxidation in heart and muscle early in life appear to be accompanied by a concurrent upregulation in the rates of fatty acid transport and the expression of FAT/CD36 in heart and muscle, as well as an increase in plasmalemmal FAT/CD36 and FABPpm in the heart, and only plasmalemmal FAT/CD36 in soleus muscle. We speculate that the rapid upregulation of fatty acid transport rates in heart and muscle are needed to support the increased rates of fatty oxidation that have been previously observed in these tissues.
在出生后的早期阶段，大鼠心脏和骨骼肌中的脂肪酸氧化速率迅速增加。因此，我们在心脏和比目鱼肌中研究了：（a）在生命早期（第10 - 36天）及之后（第84、160、365天），脂肪酸转运蛋白（FAT/CD36、FABPpm）的mRNA和蛋白质表达是否有快速变化；（b）脂肪酸转运速率以及FAT/CD36和FABPpm的质膜含量是否发生改变。在心脏（仅FAT/CD36，增加21%，P < 0.05）和比目鱼肌（FAT/CD36增加100%，P < 0.05；FABPpm减少20%，P < 0.05）中，从第10天到36天蛋白质表达迅速改变，此后无进一步变化（P < 0.05）。心脏（第10天对比第160天）和肌肉中的脂肪酸转运速率增加（分别增加33%，P < 0.05和85%，P < 0.05），并且与心脏中质膜FABPpm（增加44%，P < 0.05）和FAT/CD36（增加16%，P < 0.05）以及肌肉中仅质膜FAT/CD36（增加90%，P < 0.05）的相应增加相关。因此，已知的生命早期心脏和肌肉中脂肪酸氧化速率的变化似乎伴随着脂肪酸转运速率以及心脏和肌肉中FAT/CD36表达的同时上调，以及心脏中质膜FAT/CD36和FABPpm的增加，比目鱼肌中仅质膜FAT/CD36增加。我们推测，心脏和肌肉中脂肪酸转运速率的快速上调是为了支持先前在这些组织中观察到的脂肪酸氧化速率的增加。
