Fan Hongbin, Hu Yunyu, Zhang Chunli, Li Xusheng, Lv Rong, Qin Ling, Zhu Rui
Institute of Orthopaedics & Traumatology, Xijing Hospital, the Fourth Military Medical University, PR China.
Biomaterials. 2006 Sep;27(26):4573-80. doi: 10.1016/j.biomaterials.2006.04.013. Epub 2006 May 23.
The study was to produce a novel hybrid poly-(lactic-co-glycolic acid) (PLGA)-gelatin/chondroitin/hyaluronate (PLGA-GCH) scaffold and evaluate its potentials in cartilage repair. The porous PLGA-GCH scaffold was developed to mimic the natural extra cellular matrix of cartilage. The differentiated mesenchymal stem cells (MSCs) seeded on PLGA-GCH or PLGA scaffold were incubated in vitro and showed that, compared to PLGA scaffold, the PLGA-GCH scaffold significantly augmented the proliferation of MSCs and GAG synthesis. Then autologous differentiated MSCs/PLGA-GCH was implanted to repair full-thickness cartilage defect in rabbit, while MSCs/PLGA for the contra lateral cartilage defect (n=30). Fifteen additional rabbits without treatment for defects were used as control. Histology observation showed the MSCs/PLGA-GCH repair group had better chondrocyte morphology, integration, continuous subchondral bone, and much thicker newly formed cartilage compared with MSCs/PLGA repair group 12 and 24 weeks postoperatively. There was a significant difference in histological grading score between these two groups, which both showed much better repair than control. The present study implied that the hybrid PLGA-GCH scaffold might serve as a new way to keep the differentiation of MSCs for enhancing cartilage repair.
本研究旨在制备一种新型的聚（乳酸-乙醇酸）共聚物（PLGA）-明胶/软骨素/透明质酸盐（PLGA-GCH）混合支架，并评估其在软骨修复中的潜力。开发多孔PLGA-GCH支架以模拟软骨的天然细胞外基质。将接种在PLGA-GCH或PLGA支架上的分化间充质干细胞（MSCs）进行体外培养，结果显示，与PLGA支架相比，PLGA-GCH支架显著促进了MSCs的增殖和糖胺聚糖（GAG）的合成。然后将自体分化的MSCs/PLGA-GCH植入兔全层软骨缺损处进行修复，而将MSCs/PLGA用于对侧软骨缺损（n=30）。另外15只未经治疗的缺损兔用作对照。组织学观察显示，术后12周和24周时，MSCs/PLGA-GCH修复组的软骨细胞形态、整合情况、连续的软骨下骨以及新形成的软骨厚度均优于MSCs/PLGA修复组。这两组的组织学分级评分存在显著差异，且均显示出比对照组更好的修复效果。本研究表明，PLGA-GCH混合支架可能是一种保持MSCs分化以增强软骨修复的新方法。
