Ding Xinghong, Xia Liming
Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 310027, PRChina.
Appl Biochem Biotechnol. 2006 Jun;133(3):263-70. doi: 10.1385/abab:133:3:263.
The effects of different aeration conditions on xylitol production from corncob hemicellulose hydrolysate by Candida sp. ZU04 were investigated. Batch fermentations were carried out in a 3.7-L fermentor at 30 degrees C, pH 5.5, and agitation of 300 rpm. It was found that the two-phase aeration process was more effective than the one-phase aeration process in xylitol production. In the first 24 h of the aerobic phase, a high aeration rate was applied, glucose was soon consumed, and biomass increased quickly. In the second fermentation phase, aeration rate was reduced and an improved xylitol yield was obtained. The maximum xylitol yield (0.76 g/g) was obtained with an aeration rate of 1.5 vvm (KLa of 37 h-1) for the first 24 h and 0.3 vvm (KLa of 6 h-1) from 24 to 96 h.
研究了不同通气条件对假丝酵母属ZU04利用玉米芯半纤维素水解物生产木糖醇的影响。在3.7升发酵罐中于30℃、pH 5.5和300转/分钟的搅拌速度下进行分批发酵。结果发现，在木糖醇生产中，两相通气过程比单相通气过程更有效。在好氧阶段的前24小时，采用高通气速率，葡萄糖很快被消耗，生物量迅速增加。在第二个发酵阶段，通气速率降低，木糖醇产量提高。在最初24小时通气速率为1.5 vvm（体积传质系数KLa为37 h-1），24至96小时通气速率为0.3 vvm（KLa为6 h-1）时，获得了最大木糖醇产量（0.76克/克）。
