Zhang Feng, Brooks Darrell, Chen Weijia, Mustain William, Chen Michael B, Lineaweaver William C
Division of Plastic Surgery, University of Mississippi Medical Center, Jackson, MS 39216, USA.
Ann Plast Surg. 2006 Jun;56(6):670-3. doi: 10.1097/01.sap.0000203998.37851.57.
Expression of vascular endothelial growth factor (VEGF) in the venous flap and the effect of exogenous VEGF on survival of the venous flap were studied in rats. A 4- x 4-cm groin type 2 venous skin flap was used in the study. In part 1, biopsies were taken from the flap at 0, 6, 12, 24, and 48 hours after the flaps were raised. VEGF gene expression was measured. In part 2, exogenous VEGF (1 microg/mL) was injected subdermally into the flaps in 10 rats before the flaps were replaced. Flaps that received a saline injection were used as the control. Skin paddle survival was measured on postoperative day 7. The results showed that VEGF expression was significantly increased at 24 and 48 hours after venous flap elevation (P < 0.05). Injection of exogenous VEGF to the flap significantly improved survival of the flap (73% of the flap) when compared with the control, which had a 39% mean percent survival (P < 0.05). We conclude that VEGF expression was increased in the venous flap. Administration of exogenous VEGF significantly improved survival of the venous flap.
在大鼠中研究了血管内皮生长因子（VEGF）在静脉皮瓣中的表达以及外源性VEGF对静脉皮瓣存活的影响。本研究采用4×4cm腹股沟2型静脉皮瓣。在第1部分中，在皮瓣掀起后0、6、12、24和48小时从皮瓣取材进行活检，检测VEGF基因表达。在第2部分中，在10只大鼠的皮瓣回植前皮下注射外源性VEGF（1μg/mL），以注射生理盐水的皮瓣作为对照。术后第7天测量皮瓣存活情况。结果显示，静脉皮瓣掀起后24和48小时VEGF表达显著增加（P<0.05）。与平均存活率为39%的对照组相比，向皮瓣注射外源性VEGF显著提高了皮瓣存活率（皮瓣存活率为73%）（P<0.05）。我们得出结论，静脉皮瓣中VEGF表达增加，给予外源性VEGF可显著提高静脉皮瓣存活率。
