Tang Hua, Araki Kimi, Yamamura Ken-ichi
Key Laboratory of Molecular Biology on Infectious Diseases, Chongqing University of Medical Sciences, China.
Yi Chuan Xue Bao. 2006 May;33(5):413-9. doi: 10.1016/s0379-4172(06)60068-1.
We used gene trapping vector PU8 to search some interesting genes which play important roles in mouse development from murine ES cells. One positive ES colony termed Ayu17-449 was trapped. Its partial cDNA was obtained by using 5' RACE method. It is homologous to a 5,523 bp cDNA fragment (GI: 20879412) in EST database. Further analysis of the 5,523 bp cDNA sequence in Celera mouse gene database showed that it overlaps two genes. We designed serials of DNA primers according to the mRNAs of these two genes for RT-PCR and Northern blotting analysis, and identified a novel RNA about 9 kb (we named it as Ayu17-449) encoding 1,920 aa. This gene is expressed highly in the brain, kidney, heart, lung, muscle and stomach. The expressed protein contains a Granin motif on its N-terminus, showing that this gene may be involved in hormone secretion.
我们使用基因捕获载体PU8从鼠胚胎干细胞中寻找在小鼠发育中起重要作用的一些有趣基因。捕获到一个名为Ayu17 - 449的阳性胚胎干细胞集落。通过5' RACE方法获得了其部分cDNA。它与EST数据库中一个5523 bp的cDNA片段（GI: 20879412）同源。对Celera小鼠基因数据库中5523 bp cDNA序列的进一步分析表明，它与两个基因重叠。我们根据这两个基因的mRNA设计了一系列DNA引物用于RT-PCR和Northern印迹分析，并鉴定出一个约9 kb的新RNA（我们将其命名为Ayu17 - 449），其编码1920个氨基酸。该基因在脑、肾、心、肺、肌肉和胃中高表达。所表达的蛋白质在其N端含有一个嗜铬粒蛋白基序，表明该基因可能参与激素分泌。
