Tachi Masahiro, Hirabayashi Shinichi, Yonehara Yoshiyuki, Suzuki Yasutoshi, Bowler Philip
Department of Plastic and Reconstructive Surgery, Teikyo University School of Medicine, Tokyo, Japan.
Int Wound J. 2004 Apr;1(1):49-55. doi: 10.1111/j.1742-481x.2004.00006.x.
A model of infected skin ulceration could prove useful in assessing the clinical effectiveness of antimicrobial ointments and dressings. However, no such models have been previously established. Three types of wound were induced in rats: full-thickness wounds covered with gauze, burn wounds and wounds resulting from mechanical trauma. Wounds were inoculated with S. aureus or P. aeruginosa. Persistent infected wounds were observed only in full-thickness wounds covered with gauze. In a second experiment, colonies of P. aeruginosa or S. aureus were counted within 15 x 15 mm full-thickness wounds covered with gauze. Wounds were inoculated with 1.0 x 10(6) colony-forming units (CFU) of P. aeruginosa or S. aureus and then sealed to ensure an enclosed environment. Tissue bacterial counts exceeded 10(6) CFU/g from the next day until day 9 after infection. Bacterial counts exceeded 10(8) CFU/ml in wound exudate collected between days 1 and 7. We have developed a model of wound infection in which persistence of infection can be achieved for 9 days following ulceration due to the application of gauze to the base of a full-thickness wound.
感染性皮肤溃疡模型可能有助于评估抗菌软膏和敷料的临床效果。然而，此前尚未建立此类模型。在大鼠身上诱导出三种类型的伤口：覆盖纱布的全层伤口、烧伤伤口和机械创伤导致的伤口。伤口接种金黄色葡萄球菌或铜绿假单胞菌。仅在覆盖纱布的全层伤口中观察到持续性感染伤口。在第二个实验中，对覆盖纱布的15×15毫米全层伤口内的铜绿假单胞菌或金黄色葡萄球菌菌落进行计数。伤口接种1.0×10(6)个铜绿假单胞菌或金黄色葡萄球菌菌落形成单位（CFU），然后密封以确保封闭环境。从感染后的第二天到第9天，组织细菌计数超过10(6) CFU/g。在第1天至第7天收集的伤口渗出液中，细菌计数超过10(8) CFU/ml。我们已经开发出一种伤口感染模型，通过在全层伤口底部应用纱布，溃疡后感染可持续9天。
