猪玻璃体内注射替奈普酶的视网膜穿透性研究。
A study of retinal penetration of intravitreal tenecteplase in pigs.
作者信息
Kwan Anthony S L, Vijayasekaran Sarojini, McAllister Ian L, Yu Paula K, Yu Dao-Yi
机构信息
Lions Eye Institute, Centre for Ophthalmology and Visual Science, the University of Western Australia, Nedlands, Australia.
出版信息
Invest Ophthalmol Vis Sci. 2006 Jun;47(6):2662-7. doi: 10.1167/iovs.05-1019.
PURPOSE
To determine the degree of retinal penetration of intravitreal tenecteplase in a porcine model.
METHODS
Fluorescence-tagged tenecteplase (50 microg in 0.1 mL physiologic saline) was injected into the vitreous of the right and left eyes at 24 and 6 hours before death, respectively, in six nonvitrectomized pigs. Retinal penetration was assessed on frozen sections by epifluorescence microscopy and statistical analysis was performed. Frozen sections of two eyes without injection (control) were also assessed.
RESULTS
Labeling of fluorescence-tagged tenecteplase was seen in all the layers of the retina at both time points with more intense signal at 24 hours after injection (P<0.05).
CONCLUSIONS
Fluorescence-tagged tenecteplase can penetrate all the layers of the retina of porcine eyes after intravitreal injection. Intravitreal tenecteplase may be useful in the in the management of subretinal hemorrhage.
目的
在猪模型中确定玻璃体内注射替奈普酶后视网膜的穿透程度。
方法
分别在6只未行玻璃体切割术的猪死亡前24小时和6小时，将荧光标记的替奈普酶（50微克溶于0.1毫升生理盐水中）注入右眼和左眼玻璃体。通过落射荧光显微镜对冰冻切片进行视网膜穿透评估并进行统计分析。还对两只未注射的眼睛（对照）的冰冻切片进行了评估。
结果
在两个时间点，视网膜的所有层均可见荧光标记的替奈普酶标记，注射后24小时信号更强（P<0.05）。
结论
玻璃体内注射荧光标记的替奈普酶后可穿透猪眼视网膜的所有层。玻璃体内注射替奈普酶可能有助于治疗视网膜下出血。
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