Tu Junfang, Wang Xingming, Liu Dengyi, Li Zheng
Provincial Key Laboratory of Conservation and Exploitation of Biological Resources in Anhui, Biodiversity Research Center, Anhui Normal University, Wuhu 241000, China.
Ying Yong Sheng Tai Xue Bao. 2006 Mar;17(3):502-6.
The study with water culture showed that when treated with 0.056 mg Cu x L(-1), the chlorophyll a, b and (a + b) contents in Spirodela polyrrhiza and Lemna minor were increased by 11%, 46% and 22%, and 8%, 15% and 11%, while under 0.15 to approximately 5.6 mg Cu x L(-1) the contents were decreased by 63%, 62% and 63%, and 46%, 45% and 45%, respectively, compared with no copper added. The malondialdehyde (MDA) content in S. polyrrhiza was 2.75 times higher than that in L. minor averagely. The superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities in both S. polyrrhiza and L. minor all increased first, but decreased then with increasing copper concentration. For S. polyrrhiza, the activities of test enzymes were the highest at 0.18 mg Cu-L(-1), but for L. minor, only when the Cu concentrations were up to 0.56, 1.0 and 1.0 mg x L(-1), the activities of SOD, CAT and POD got to their peak values, respectively. It was concluded that higher concentrations of copper caused more damage to S. polyrrhiza than to L. minor, and the antioxidase system of the former was much more sensitive to the copper stress than that of the latter.
水培实验表明，当用0.056毫克铜/升处理时，紫萍和浮萍中的叶绿素a、b及（a + b）含量分别增加了11%、46%和22%，以及8%、15%和11%；而在0.15至约5.6毫克铜/升的浓度下，与不添加铜相比，其含量分别下降了63%、62%和63%，以及46%、45%和45%。紫萍中的丙二醛（MDA）含量平均比浮萍高2.75倍。紫萍和浮萍中的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性均先升高，但随后随着铜浓度的增加而下降。对于紫萍，测试酶的活性在0.18毫克铜/升时最高；而对于浮萍，只有当铜浓度分别达到0.56、1.0和1.0毫克/升时，SOD、CAT和POD的活性才分别达到峰值。研究得出结论，较高浓度的铜对紫萍造成的损害比对浮萍更大，且前者的抗氧化酶系统对铜胁迫比后者更为敏感。
