Hirst P J, Demayo F J, Dukelow W R
Theriogenology. 1981 Jan;15(1):67-75. doi: 10.1016/s0093-691x(81)80019-2.
Xenogenous fertilization was accomplished using bovine, porcine, and hamster follicular oocytes. The xenogenous fertilization rates for bovine and porcine follicular oocytes in the oviduct of the pseudopregnant rabbit were 13.4% and 2.0%, respectively. Temperatures of ovary, during transport to the laboratory, of 0 degrees or 37 degrees C had no effect on xenogenous fertilization rates of bovine oocytes. In vitro culture in 50 mug/ml FSH did not alter the xenogenous fertilization rates of bovine oocytes. Fertilization was observed with oocytes recovered 40 to 75 hr after insemination. Two cell embryos were recovered 70 to 75 hr after insemination. Ligation of the rabbit oviduct, number of ova deposited and sperm concentration did not affect the xenogenous fertilization rates of hamster ova. Cleavage of xenogenously fertilized hamster oocytes occurred between 28 and 29 hours after insemination.
采用牛、猪和仓鼠卵泡卵母细胞完成了异种受精。在假孕兔输卵管中，牛和猪卵泡卵母细胞的异种受精率分别为13.4%和2.0%。在运往实验室的过程中，卵巢温度为0℃或37℃对牛卵母细胞的异种受精率没有影响。在50微克/毫升促卵泡素中进行体外培养并未改变牛卵母细胞的异种受精率。在授精后40至75小时回收的卵母细胞中观察到了受精现象。在授精后70至75小时回收了二细胞胚胎。兔输卵管结扎、植入的卵子数量和精子浓度不影响仓鼠卵子的异种受精率。异种受精的仓鼠卵母细胞在授精后28至29小时之间发生卵裂。
