Archbald L F, Al-Bagdadi F, Godke R A
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
Theriogenology. 1981 Jul;16(1):27-37. doi: 10.1016/0093-691x(81)90110-2.
Corpora lutea were obtained surgically from fifteen mature Angus crossbred cows representing three experimental groups of five cows each. Cows in Group A were 180 days of gestation, cows in Group B had recently experienced parturition (<or=24 hours) and cows in Group C had recently exhibited standing estrus (<or=24 hours). Samples of corpora lutea were fixed in 10% buffered formalin and prepared for light microscopy and 2.5% glutaraldehyde and processed for electron microscopy. Luteal cells from cows in Group A were of two distinct types, large cell and small cell types. The large luteal cells of both Groups A and B were similar, with one exception. Those from cows in Group B stained less intensely and the nuclear chromatin appeared less pronounced and marginated. Small luteal cells were not readily observed in corpora lutea from Group B. In addition, there was evidence of pyknosis, karyorrhexis and mononuclear cell infiltration in corpora lutea from Group B. Luteal cells from cows in Group C were characterized by atrophy of the nucleus and vacuolation of the cytoplasm. Vascular changes consisted of endothelial cell necrosis and pyknosis of cells of the tunica media of arteries within the corpus luteum. Ultrastructurally, there were distinct differences in all groups with respect to the size and quantity of lipid droplets, the presence of electron lucent vesicles in the cytoplasm and indentations of the nuclear membrane of luteal cells. The results of this study suggest that at the time of parturition in the cow luteolysis may involve primarily small luteal cells, and that the corpus luteum may be a source of progesterone, which is likely a function of the large luteal cells.
从15头成熟的安格斯杂交奶牛身上通过手术获取黄体，这些奶牛分为三个实验组，每组5头。A组奶牛处于妊娠180天，B组奶牛最近经历了分娩（≤24小时），C组奶牛最近表现出站立发情（≤24小时）。黄体样本用10%缓冲福尔马林固定，制备用于光学显微镜观察，并用2.5%戊二醛处理以用于电子显微镜观察。A组奶牛的黄体细胞有两种不同类型，即大细胞型和小细胞型。A组和B组的大黄体细胞相似，但有一个例外。B组奶牛的大黄体细胞染色较浅，核染色质不那么明显且边缘化。在B组的黄体中不容易观察到小黄体细胞。此外，有证据表明B组黄体出现核固缩、核碎裂和单核细胞浸润。C组奶牛的黄体细胞特征为细胞核萎缩和细胞质空泡化。血管变化包括内皮细胞坏死和黄体中动脉中膜细胞的核固缩。在超微结构上，所有组在脂滴的大小和数量、细胞质中电子透明小泡的存在以及黄体细胞核膜的凹陷方面存在明显差异。这项研究的结果表明，在奶牛分娩时，黄体溶解可能主要涉及小黄体细胞，并且黄体可能是孕酮的来源，这可能是大黄体细胞的功能。
