Linares T, Larsson K, Edqvist L E
Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences, S-750 07 Uppsala, Sweden.
Theriogenology. 1982 Feb;17(2):125-32. doi: 10.1016/0093-691x(82)90072-3.
Twenty-five dairy heifers, 11 repeat breeder and 14 virgin heifers, were used. Blood samples were taken twice a day (9 a.m. and 3 p.m.) from day of A.I. to 7 days later when embryo collection was made. One series of blood samples were taken from 16 heifers and two and three, respectively, from 6 and 3 heifers. Analysis of progesterone was performed by radioimmunoassay. The embryos were classified in 3 groups: normal blastocysts, morphological deviators, and degenerated embryos. Analysis of variance and a discriminant analysis was performed. The discriminant analysis was made to predict embryonic morphology, using daily progesterone values as predictors. No significant relationship between embryonic morphology and progesterone levels was found. The discriminant analysis showed that 64.9 % of progesterone curves were correctly classified, with individual groups ranging from 50 % (degenerated) to 76.5 % (normal embryos). It was concluded that abnormal embryonic development occurs with normal lateal function and that normal bovine embryo does not exhibit luteotrophic activity 7 days after mating.
使用了25头奶牛小母牛，其中11头为屡配不孕母牛，14头为初产小母牛。从人工授精当天至7天后进行胚胎采集期间，每天采集两次血样（上午9点和下午3点）。分别从16头小母牛采集了一组血样，从6头和3头小母牛采集了两组和三组血样。采用放射免疫分析法测定孕酮。胚胎分为3组：正常囊胚、形态异常胚胎和退化胚胎。进行了方差分析和判别分析。判别分析以每日孕酮值为预测指标来预测胚胎形态。未发现胚胎形态与孕酮水平之间存在显著关系。判别分析表明，64.9%的孕酮曲线分类正确，各单独组的分类正确率从50%（退化胚胎）到76.5%（正常胚胎）不等。得出的结论是，在黄体功能正常的情况下会出现异常胚胎发育，并且正常的牛胚胎在配种7天后不表现出促黄体活性。
