Mizinga K M, Verma O P
Department of Physiology and Pharmacology School of Veterinary Medicine Tuskegee Institute, AL 36088 USA.
Theriogenology. 1984 Mar;21(3):435-46. doi: 10.1016/0093-691x(84)90405-9.
Seventeen female mature anestrous does were used to study the effect of luteinizing hormone-releasing hormone (LHRH) on ovulation (Experiment I) and on fertility rate and blood estrogen/progesterone concentrations (Experiment II). Laparotomy after day 8 of treatment with a single injection of LHRH (300 ug) and estradiol cypionate (0.2 mg/kg) revealed evidence of ovulation in two out of three and a developed follicle in the third. Similar treatment to six does in Experiment II, when followed by natural mating with a fertile buck, produced pregnancy in two does, pseudopregnancy in two and no effect (nonpregnancy) in the remaining two animals. The pregnant does had normal parturition after 148 to 150 days of gestation. In pregnant does, blood progesterone levels first showed a gradual increase until day 130 (10.07 ng/ml) and then declined sharply at 48 hours before parturition. Estrogen concentration, on the other hand, failed to increase until day 80, and thereafter it reached a peak (1800 pg/ml) at 24 hours prior to parturition; the level declined sharply at 24 hours after parturition. In pseudopregnant does, progesterone levels remained in close proximity with those of pregnant does until day 90, when they started to decline.
选用17只处于乏情期的成年雌性兔来研究促黄体生成激素释放激素（LHRH）对排卵的影响（实验I）以及对生育率和血液中雌激素/孕激素浓度的影响（实验II）。在单次注射LHRH（300微克）和环丙孕酮（0.2毫克/千克）治疗8天后进行剖腹手术，结果显示三只兔子中有两只出现排卵迹象，第三只兔子有一个发育成熟的卵泡。在实验II中，对六只兔子进行类似治疗，随后与一只可育雄兔自然交配，两只兔子怀孕，两只假孕，其余两只无效果（未怀孕）。怀孕的兔子在妊娠148至150天后正常分娩。在怀孕的兔子中，血液中的孕酮水平在第130天前逐渐升高至10.07纳克/毫升，然后在分娩前48小时急剧下降。另一方面，雌激素浓度直到第80天才开始升高，此后在分娩前24小时达到峰值（1800皮克/毫升）；分娩后24小时水平急剧下降。在假孕的兔子中，孕酮水平在第90天前与怀孕兔子的水平相近，之后开始下降。
