多氯联苯对原代培养大鼠肝细胞胆固醇和抗坏血酸代谢的影响。

Effects of polychlorinated biphenyls on cholesterol and ascorbic acid metabolism in primary cultured rat hepatocytes.

作者信息

Nagaoka S, Kamuro H, Oda H, Yoshida A

机构信息

Department of Agricultural Chemistry, Nagoya University-Nagoya, Japan.

出版信息

Biochem Pharmacol. 1991 Apr 15;41(8):1259-61. doi: 10.1016/0006-2952(91)90667-t.

DOI:10.1016/0006-2952(91)90667-t

PMID:1672595

Abstract

Urea formation, cell number, cellular protein content and tyrosine transaminase activity were not changed by PCB addition in cultured rat hepatocytes. These results indicate that these liver functions are similar to control levels despite the addition of PCBs. Our results show that primary cultured hepatocytes mimic partially the intact liver in relation to some responses to PCBs. In isolated hepatocytes as well as in vivo, PCBs increased in cellular ascorbic acid level, aryl hydrocarbon hydroxylase and UDP-glucuronyl transferase activities. Thus, the present results may suggest that the metabolic changes in vivo concerning ascorbic acid induced by dietary PCBs were primarily related to the direct effects of PCBs per se on liver parenchymal cells.

摘要

在培养的大鼠肝细胞中添加多氯联苯（PCB）后，尿素生成、细胞数量、细胞蛋白质含量和酪氨酸转氨酶活性均未发生变化。这些结果表明，尽管添加了多氯联苯，但这些肝功能仍与对照水平相似。我们的结果表明，原代培养的肝细胞在对多氯联苯的某些反应方面部分模拟了完整肝脏。在分离的肝细胞以及体内，多氯联苯会使细胞内抗坏血酸水平、芳烃羟化酶和UDP-葡糖醛酸基转移酶活性增加。因此，目前的结果可能表明，饮食中的多氯联苯在体内引起的与抗坏血酸有关的代谢变化主要与多氯联苯本身对肝实质细胞的直接作用有关。