Leibfried-Rutledge M L, Critser E S, First N L
Department of Meat and Animal Science, University of Wisconsin, Madison, WI 53706, USA.
Theriogenology. 1985 May;23(5):753-9. doi: 10.1016/0093-691x(85)90150-5.
Bovine follicular oocytes, collected from two sizes of vesicular follicles and from donor animals from three stages of the estrous cycle, were matured and fertilized in vitro. Frequency of fertilization and ability to form male pronuclei after in vitro maturation were found to be independent of both estrual stage and follicular size.
从两种大小的囊状卵泡以及处于发情周期三个阶段的供体动物采集的牛卵泡卵母细胞,在体外进行成熟培养和受精。结果发现,体外成熟后受精的频率以及形成雄原核的能力与发情阶段和卵泡大小均无关。