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A semiquantitative and rapid radioimmunosorbent assay of luteinizing hormone for early prediction of time of ovulation in cattle.

作者信息

Høier R, Callesen H, Greve T

机构信息

Department of Animal Reproduction, The Royal Veterinary and Agricultural University, Copenhagen, Denmark.

出版信息

Theriogenology. 1988 Aug;30(2):235-43. doi: 10.1016/0093-691x(88)90173-2.

DOI:10.1016/0093-691x(88)90173-2
PMID:16726466
Abstract

A radioimmunoassay (RIA) for bovine luteinizing hormone (LH) is presented. It is based on the binding of the second antibody to polystyrene vials. Less than 4 h is required to estimate the hormone level in the plasma. LH content is determined by comparing unknown plasma samples to relevant control plasma to identify the preovulatory LH surge. Of 182 samples, 176 (97%) were found to contain either more or less LH than the control plasma, and 12 out of 13 (92%) LH surges were identified using the immunosorbent RIA. The method can be used for early prediction of ovulation in cattle, and it may be used to time aspiration of oocytes.

摘要

相似文献

1
A semiquantitative and rapid radioimmunosorbent assay of luteinizing hormone for early prediction of time of ovulation in cattle.
Theriogenology. 1988 Aug;30(2):235-43. doi: 10.1016/0093-691x(88)90173-2.
2
Prediction of human ovulation by rapid luteinizing hormone (LH) radioimmunoassay and ovarian ultrasonography.通过快速促黄体生成素(LH)放射免疫测定和卵巢超声检查预测人类排卵
Fertil Steril. 1982 Aug;38(2):194-201. doi: 10.1016/s0015-0282(16)46459-9.
3
Insights into the mechanism by which kisspeptin stimulates a preovulatory LH surge and ovulation in seasonally acyclic ewes: potential role of estradiol.揭示 kisspeptin 刺激季节性非发情绵羊发情和排卵的机制:雌二醇的潜在作用。
Domest Anim Endocrinol. 2010 May;38(4):289-98. doi: 10.1016/j.domaniend.2010.01.001. Epub 2010 Jan 16.
4
Impairment in follicle-stimulating hormone secretion by different treatments with follicular fluid as a source of inhibin blocks the ability of pulsatile luteinizing hormone to induce preovulatory surges of gonadotropins in prepubertal ewe lambs.以卵泡液作为抑制素来源进行不同处理,会损害促卵泡激素的分泌,从而阻断脉冲式促黄体生成素诱导青春期前母羊羔羊促性腺激素排卵前激增的能力。
Biol Reprod. 1996 Feb;54(2):387-93. doi: 10.1095/biolreprod54.2.387.
5
Testosterone antagonist (flutamide) blocks ovulation and preovulatory surges of progesterone, luteinizing hormone and oestradiol in laying hens.睾酮拮抗剂(氟他胺)可阻断蛋鸡的排卵以及孕酮、促黄体生成素和雌二醇的排卵前激增。
Reproduction. 2006 Jun;131(6):1109-14. doi: 10.1530/rep.1.01067.
6
Onset and characteristics of the midcycle surge in bioactive and immunoactive luteinizing hormone secretion in normal women: influence of physiological variations in periovulatory ovarian steroid hormone secretion.正常女性生物活性和免疫活性促黄体生成素分泌的月经周期中期高峰的起始与特征:排卵期卵巢甾体激素分泌的生理变化的影响
J Clin Endocrinol Metab. 1992 Aug;75(2):489-93. doi: 10.1210/jcem.75.2.1639949.
7
Changes in luteinizing hormone-releasing hormone content of discrete hypothalamic areas associated with spontaneous and induced preovulatory luteinizing hormone surges in the domestic hen.家鸡下丘脑离散区域促黄体生成素释放激素含量的变化与自发和诱导的排卵前促黄体生成素激增相关。
Biol Reprod. 1985 May;32(4):813-9. doi: 10.1095/biolreprod32.4.813.
8
Assessing the preovulatory LH surge with a three-hour radioimmunoassay.采用三小时放射免疫测定法评估排卵前促黄体生成素激增。
J Reprod Med. 1983 Jun;28(6):402-6.
9
On differences between the preovulatory luteinizing hormone surges of 4- and 5-day cyclic rats.关于4日和5日周期大鼠排卵前促黄体生成素激增的差异
J Endocrinol. 1976 Sep;70(3):373-8. doi: 10.1677/joe.0.0700373.
10
Prediction of the time of ovulation.排卵时间的预测。
Fertil Steril. 1981 Sep;36(3):308-15.

引用本文的文献

1
In vivo development of in vitro fertilized bovine oocytes matured in vivo versus in vitro.体内成熟与体外成熟的体外受精牛卵母细胞的体内发育情况
J In Vitro Fert Embryo Transf. 1987 Oct;4(5):281-5. doi: 10.1007/BF01555205.
2
A comparative ultrastructural study of in vivo versus in vitro fertilization of bovine oocytes.牛卵母细胞体内受精与体外受精的超微结构比较研究
Anat Embryol (Berl). 1989;179(5):435-42. doi: 10.1007/BF00319585.