在33摄氏度和37摄氏度下进行病毒分离和滴定。

Virus isolation and titration at 33 degrees and 37 degrees C.

作者信息

Fuchs N, Wigand R

出版信息

Med Microbiol Immunol. 1975;161(2):123-6. doi: 10.1007/BF02121753.

DOI:10.1007/BF02121753

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7086892/

Abstract

Various prototype viruses and original specimens were comparatively titrated in cell cultures at 33 degrees and 37 degrees C. Higher titers at 37 degrees were consistently obtained with adenoviruses; for other viruses (enteroviruses, herpesvirus hominis, vaccinia virus, parainfluenza viruses) the titers were mostly identical at either temperature. Original specimens and prototye strains showed the same behavior. The habit to cultivate viruses from throat swabs at 33 degrees C is unsatisfactory for adenoviruses.

摘要

在33摄氏度和37摄氏度的细胞培养物中对各种原型病毒和原始标本进行了比较滴定。腺病毒在37摄氏度时始终能获得更高的滴度；对于其他病毒（肠道病毒、人疱疹病毒、牛痘病毒、副流感病毒），在这两种温度下的滴度大多相同。原始标本和原型菌株表现出相同的行为。在33摄氏度下从咽拭子培养病毒的习惯对于腺病毒来说并不理想。

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[Laboratory procedures in adenoviruses. II. Sensitivity of various cell cultures as determined by endpoint titration (author's transl)].[腺病毒的实验室操作。II. 通过终点滴定法测定的各种细胞培养物的敏感性（作者译）]

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