Staley A L, Rinehart K L
Roger Adams Laboratory, University of Illinois, Urbana-Champaign 61801.
J Antibiot (Tokyo). 1991 Feb;44(2):218-24. doi: 10.7164/antibiotics.44.218.
[Carboxy-14C]-3-amino-5-hydroxybenzoic acid (AHBA) has been shown to be incorporated by Streptomyces spectabilis to the extent of greater than 0.1% (35: 1 dilution) in the ansamycin antibiotic streptovaricin C, the major component of the streptovaricin complex. When [carboxy-13C]AHBA was similarly administered, C-21 (quinone methide carbonyl at 188.3 ppm) of streptovaricin C was specifically labeled (at twenty one times natural abundance). In preparation for the 13C incorporation study the 13C NMR spectrum of streptovaricin C was investigated, making extensive use of short- and long-range HETCOR. These assignments revise some of those proposed earlier for streptovaricin C.
[羧基-¹⁴C]-3-氨基-5-羟基苯甲酸（AHBA）已被证明可被壮观链霉菌整合到安莎霉素抗生素链黑菌素C中，其整合程度大于0.1%（35:1稀释），链黑菌素C是链黑菌素复合物的主要成分。当以类似方式施用[羧基-¹³C]AHBA时，链黑菌素C的C-21（188.3 ppm处的醌甲基羰基）被特异性标记（为自然丰度的21倍）。在准备¹³C整合研究时，对链黑菌素C的¹³C NMR谱进行了研究，广泛使用了短程和长程异核多量子相干谱。这些归属修正了一些先前对链黑菌素C提出的归属。
