Kamimura S, Enomoto S, Goto K, Hamana K
School of Veterinary Medicine, Faculty of Agriculture Kagoshima University, Kagoshima 890, Japan.
Theriogenology. 1993 Oct;40(4):853-8. doi: 10.1016/0093-691x(93)90220-y.
A Globosus amorphus along with a living calf was encountered following the transfer of blastocysts obtained by in vitro fertilization of in vitro-matured follicular oocytes in Japanese black cattle. Two embryos obtained 9 days after in vitro fertilization developed into either a hatched blastocyst with distinct inner cell mass or an expanded blastocyst with indistinct inner cell mass. The embryos were loaded into a 0.25-ml plastic straw and were nonsurgically transferred to the uterus of a heifer on Day 8 (Day 0 = estrus). On Day 75, a twin pregnancy was ultrasonically diagnosed in the right uterine horn, in which a live fetus with distinct limbs and a concomitant ovoid mass were detected. On Day 287, the dam developed parturient paralysis with dropsy of the fetal membranes. By palpation per rectum an ovoid mass was detected in the body of the uterus [corpus uteri] and a larger live fetus was in the uterine horn. A cesarean section was performed to extract a live fetus and a Globosus amorphus. The live fetus was female with the 60, XX female complements.
在对日本黑牛体外成熟卵泡卵母细胞进行体外受精获得囊胚并进行移植后，发现了一个球形畸胎瘤和一头活小牛。体外受精9天后获得的两个胚胎发育成了具有明显内细胞团的孵化囊胚或内细胞团不明显的扩张囊胚。将胚胎装入0.25毫升的塑料细管中，并在第8天（第0天=发情期）通过非手术方式移植到一头小母牛的子宫内。在第75天，超声诊断出右侧子宫角怀有双胎，其中检测到一个四肢清晰的活胎儿和一个伴随的卵圆形肿块。在第287天，母牛出现产褥期麻痹并伴有胎膜水肿。通过直肠触诊，在子宫体[子宫体]中检测到一个卵圆形肿块，子宫角中有一个较大的活胎儿。进行剖宫产以取出一个活胎儿和一个球形畸胎瘤。活胎儿为雌性，具有60, XX的雌性染色体组成。
