Kumi-Diaka J, Badtram G
Department of Medical Sciences, School of Veterinary Medicine University of Wisconsin, Madison, WI, USA.
Theriogenology. 1994;41(7):1355-66. doi: 10.1016/0093-691x(94)90187-n.
The effect of storage of canine semen on sperm membrane integrity, as determined by the hypoosmotic swelling test, and on other functional characteristics of the canine spermatozoa was evaluated by established procedures. The results of this study indicated that storage of canine semen at a chilling temperature of 5 degrees C for 24 h did not significantly impair the physical and functional characteristics of the canine spermatozoa. The overall mean percentage of motility, hypo-osmotic swelling response, which assessed sperm membrane integrity, acrosome-reacted spermatozoa, acrosomal defects, and the percentage of live spermatozoa, did not significantly differ between the fresh and chilled semen samples. However, storage altered the rate of motility and acrosome reaction. The percentage of acrosome reaction in the canine capacitating medium peaked earlier in chilled than in fresh semen. It is probable that storing semen at 5 degrees C initiated/triggered the acrosome reaction. This did not amount to impairment of functional properties. Significant correlations were observed between hypo-osmotic swelling vs motility (r=0.98, P<0.002); hypo-osmotic swelling vs acrosome reaction (r=0.83, P<0.08); and acrosome reaction vs motility (R=0.89, P<0.04) in the fresh semen, and between hypo-osmotic swelling vs motility (r=0.87, P<0.05) and hypo-osmotic swelling vs acrosome reaction (r=0.56, P<0.05) in the chilled semen. It was concluded: that 1) storage of canine semen at 5 degrees C for 24 h did not significantly impair the physical and functional integrity of the spermatozoa; 2) the significant association between motility or acrosome reaction vs hypo-osmotic swelling indicates their value in assessing sperm viability; and 3) the hypo-osmotic swelling assay could have predictive value in screening out subfertile males with apparently normal spermiograms.
通过既定程序评估了犬精液储存对精子膜完整性（由低渗肿胀试验测定）以及犬精子其他功能特性的影响。本研究结果表明，犬精液在5℃冷藏温度下储存24小时不会显著损害犬精子的物理和功能特性。新鲜精液样本和冷藏精液样本之间，活力的总体平均百分比、评估精子膜完整性的低渗肿胀反应、顶体反应精子、顶体缺陷以及活精子百分比均无显著差异。然而，储存改变了活力和顶体反应的速率。在犬获能培养基中，冷藏精液的顶体反应百分比比新鲜精液更早达到峰值。很可能在5℃储存精液引发了顶体反应。但这并不等同于功能特性受损。在新鲜精液中，低渗肿胀与活力之间（r = 0.98，P < 0.002）、低渗肿胀与顶体反应之间（r = 0.83，P < 0.08）以及顶体反应与活力之间（R = 0.89，P < 0.04）存在显著相关性；在冷藏精液中，低渗肿胀与活力之间（r = 0.87，P < 0.05）以及低渗肿胀与顶体反应之间（r = 0.56，P < 0.05）存在显著相关性。得出以下结论：1）犬精液在5℃储存24小时不会显著损害精子的物理和功能完整性；2）活力或顶体反应与低渗肿胀之间的显著关联表明它们在评估精子活力方面的价值；3）低渗肿胀试验在筛选精子图谱看似正常的亚生育雄性方面可能具有预测价值。
