Martino A, Palomo M J, Mogas T, Paramio M T
Departament of Pathology and Animal Production, Faculty of Veterinary Sciences, Autonomus University of Barcelona, Bellaterra, Barcelona, Spain.
Theriogenology. 1994 Oct;42(5):859-73. doi: 10.1016/0093-691x(94)90454-q.
Prepubertal goat ovaries obtained from a slaughterhouse were used to study the influence of the oocyte collection technique (dissection, aspiration and slicing) on the number of oocytes recovered and their capacity for maturation and fertilization in vitro. The oocytes were recovered using 3 techniques, were selected for culture and were classified according to the number of cumulus cell layers. The numbers of oocytes selected per ovary were 1.71, 1.27 and 6.05 for dissection, aspiration and slicing, respectively. The percentages of maturation obtained for slicing (56.9%) were lower than those obtained for dissection and aspiration (69.3 and 72.0%, respectively). The proportion of oocytes with the most cumulus cell layers (complete cumulus) was greatest for oocytes recovered by dissection, but this had no influence on their capacity for nuclear maturation. The total percentage of fertilization was similar for oocytes obtained by dissection and by slicing, but the latter yielded a lower percentage of normal fertilization (29.1 vs 18.2%). Of the oocytes obtained by slicing, no difference was observed in the fertilization rate between oocytes with a partial cumulus and a complete cumulus. The decrease in maturation time from 27 to 25.5 and 24 h did not improve the results for fertilization but caused a decrease in the percentage of nuclear maturation. In conclusion, the recovery of oocytes using the slicing technique yielded more oocytes per ovary than dissection or aspiration, although the in vitro fertilization capacity of oocytes obtained by the slicing method was lower than for oocytes obtained by dissection.
从屠宰场获取的青春期前山羊卵巢用于研究卵母细胞采集技术（解剖、抽吸和切片）对回收的卵母细胞数量及其体外成熟和受精能力的影响。使用3种技术回收卵母细胞，选择用于培养，并根据卵丘细胞层数进行分类。通过解剖、抽吸和切片从每个卵巢中回收的卵母细胞数量分别为1.71、1.27和6.05。切片法获得的成熟率（56.9%）低于解剖法和抽吸法（分别为69.3%和72.0%）。通过解剖回收的卵母细胞中，具有最多卵丘细胞层（完整卵丘）的卵母细胞比例最大，但这对其核成熟能力没有影响。解剖法和切片法获得的卵母细胞的总受精率相似，但后者的正常受精率较低（29.1%对18.2%）。在通过切片获得的卵母细胞中，部分卵丘和完整卵丘的卵母细胞受精率没有差异。成熟时间从27小时减少到25.5小时和24小时并没有改善受精结果，但导致核成熟率下降。总之，尽管切片法获得的卵母细胞的体外受精能力低于解剖法，但使用切片技术回收卵母细胞时每个卵巢获得的卵母细胞比解剖法或抽吸法更多。
