Rahal M D, Koo G C, Osmond D G
Department of Anatomy, McGill University, Montreal, Quebec, Canada.
Cell Immunol. 1991 Apr 15;134(1):111-25. doi: 10.1016/0008-8749(91)90335-9.
The population dynamics of "null" small lymphocytes lacking B and T lineage markers in mouse bone marrow have been examined using a combination of immunolabeling and hydroxyurea (HU) deletion techniques. The binding of the B lineage-associated mAb, 14.8, and anti-Thy1.2 to bone marrow cells has been detected radioautographically. Null cells lacking 14.8 and Thy1.2 determinants (14.8- Thy1-) formed a substantial subset (12-14%) of bone marrow small lymphocytes, representing 0.5 x 10(6) cells per femur (2-3% of nucleated cells). HU treatment revealed an exceptionally rapid turnover of the null small lymphocyte population (T1/2, 7.5 hr) compared with 14.8+ cells (T1/2, 20.5 hr) and Thy1+ cells (T1/2, 53 hr). Small lymphocytes bearing low intensities of Thy1 (Thy1lo) were also rapidly renewed (T1/2, 28 hr) whereas those with high intensities of Thy1 (Thy1hi) were renewed only slowly (T1/2, 123 hr). During ontogeny, null small lymphocytes first appeared in the fetal liver by Day 11 and the fetal spleen by Day 16, but increased rapidly in the bone marrow in early postnatal life. Double immunolabeling techniques demonstrated that 10% of null small lymphocytes in the bone marrow expressed NK1.1 antigen, while larger proportions bound to tumor (YAC.1) cells in vitro and displayed Fc receptors. The NK1.1-bearing fraction of null small lymphocytes in bone marrow was depleted by HU treatment only after an initial delay. NK1.1 was also expressed on subsets of Thy1lo cells and Thy1hi cells. The results have revealed the continuous production in mouse bone marrow of null and Thy1lo small lymphocytes, totaling 1-3 x 10(7) cells/day and 1.2 x 10(6) cells/day, respectively. The findings suggest that the large-scale production of null lymphocytes in mouse bone marrow includes the genesis of NK lineage cells which express NK1.1 and Thy1lo during a period of terminal maturation.
运用免疫标记和羟基脲（HU）缺失技术相结合的方法，对小鼠骨髓中缺乏B和T谱系标记的“无标记”小淋巴细胞的群体动力学进行了研究。通过放射自显影检测B谱系相关单克隆抗体14.8和抗Thy1.2与骨髓细胞的结合。缺乏14.8和Thy1.2决定簇（14.8 - Thy1-）的无标记细胞构成了骨髓小淋巴细胞的一个重要亚群（12 - 14%），每根股骨中有0.5×10⁶个细胞（占核细胞的2 - 3%）。与14.8⁺细胞（半衰期为20.5小时）和Thy1⁺细胞（半衰期为53小时）相比，HU处理显示无标记小淋巴细胞群体的更新速度异常快（半衰期为7.5小时）。低强度表达Thy1（Thy1lo）的小淋巴细胞更新也很快（半衰期为28小时），而高强度表达Thy1（Thy1hi）的小淋巴细胞更新则很慢（半衰期为123小时）。在个体发育过程中，无标记小淋巴细胞在第11天首次出现在胎肝中，在第16天出现在胎脾中，但在出生后早期骨髓中迅速增加。双重免疫标记技术表明，骨髓中10%的无标记小淋巴细胞表达NK1.1抗原，而更大比例的细胞在体外与肿瘤（YAC.1）细胞结合并显示Fc受体。骨髓中无标记小淋巴细胞的NK1.1阳性部分在HU处理后仅在初始延迟后才减少。NK1.1也在Thy1lo细胞和Thy1hi细胞亚群上表达。结果表明，小鼠骨髓中持续产生无标记和Thy1lo小淋巴细胞，分别总计为每天1 - 3×10⁷个细胞和每天1.2×10⁶个细胞。这些发现表明，小鼠骨髓中无标记淋巴细胞的大规模产生包括NK谱系细胞的生成，这些细胞在终末成熟阶段表达NK1.1和Thy1lo。
