Kirmizi Serap, Güleryüz Gürcan
Uludağ University, Faculty of Science and Arts, Department of Biology, Bursa, Turkey.
Z Naturforsch C J Biosci. 2006 Mar-Apr;61(3-4):222-6. doi: 10.1515/znc-2006-3-411.
The protein mobilization from attached and detached seeds of Vicia faba L. cv. Eresen 87 (Fabaceae) was investigated. While the total soluble protein content decreased, the free amino acid content increased during the 7 days germination period. Among the three proteolytic enzymes, only endopeptidase activity was found to be affected by the removal of the embryonic axis. Leucine aminopeptidase activity was high at the beginning, then it decreased; carboxypeptidase activity reached the highest value at day 5. In order to examine the effects of plant growth regulators on detached cotyledons incubated with plant growth regulators [10(-4) M benzyladenine (BA), gibberellic acid (GA3), indole acetic acid (IAA) and 10(-5) M abscisic acid (ABA)], only benzyladenine was found promotive on protein mobilization. Our results suggest that the removal of the embryonic axis in seeds of Vicia faba L. cv. Eresen 87 decreases protein mobilization and endopeptidase activity.
对蚕豆（Vicia faba L. cv. Eresen 87，豆科）附着和分离种子中的蛋白质动员进行了研究。在7天的萌发期内，总可溶性蛋白质含量下降，而游离氨基酸含量增加。在三种蛋白水解酶中，仅发现内肽酶活性受胚轴去除的影响。亮氨酸氨肽酶活性开始时较高，然后下降；羧肽酶活性在第5天达到最高值。为了研究植物生长调节剂对用植物生长调节剂[10(-4) M苄基腺嘌呤（BA）、赤霉素（GA3）、吲哚乙酸（IAA）和10(-5) M脱落酸（ABA）]处理的分离子叶的影响，仅发现苄基腺嘌呤对蛋白质动员有促进作用。我们的结果表明，去除蚕豆（Vicia faba L. cv. Eresen 87）种子中的胚轴会降低蛋白质动员和内肽酶活性。
