Kim Jiyoung, Kim Ho-Young
Kiyong Biotechnology, Saha-Gu, Busan 604-083, South Korea.
Biochim Biophys Acta. 2006 Mar-Apr;1759(3-4):191-4. doi: 10.1016/j.bbaexp.2006.03.002. Epub 2006 Mar 30.
The suppression subtractive hybridization (SSH) method was used to isolate abscisic acid (ABA)-regulated genes in Arabidopsis. We report that the AtAIG1 gene is up-regulated by ABA and the AtAIG1 encodes a basic/helix-loop-helix (bHLH)-type transcription factor in Arabidopsis. The AtAIG1 was targeted into nucleus and bound to the E-box-DNA sequence, which is found in the promoter regions of many ABA-responsive genes. Functional analyses with the ataig1 knockout mutants revealed that the mutant plants show enhanced sensitivity to ABA. In addition, the ataig1 plants showed reduced expression of ABA-responsive genes, such as RD29A and RD22. Thus, these functional analyses suggest that the AtAIG1 protein plays an important role in ABA-mediated signal transduction pathway.
采用抑制性消减杂交（SSH）方法分离拟南芥中脱落酸（ABA）调控的基因。我们报道AtAIG1基因受ABA上调，且AtAIG1在拟南芥中编码一种碱性/螺旋-环-螺旋（bHLH）型转录因子。AtAIG1定位于细胞核并与E-box-DNA序列结合，该序列存在于许多ABA响应基因的启动子区域。对ataig1敲除突变体的功能分析表明，突变植株对ABA表现出增强的敏感性。此外，ataig1植株中ABA响应基因如RD29A和RD22的表达降低。因此，这些功能分析表明AtAIG1蛋白在ABA介导的信号转导途径中起重要作用。
