Kamikawaji N, Fujisawa K, Yoshizumi H, Fukunaga M, Yasunami M, Kimura A, Nishimura Y, Sasazuki T
Department of Genetics, Kyushu University, Fukuoka, Japan.
J Immunol. 1991 Apr 15;146(8):2560-7.
We have found that the low immune response to streptococcal cell wall Ag (SCW) was inherited as a dominant trait and was linked to HLA, as deduced from family analysis. In the present report, HLA class II alleles of healthy donors were determined by serology and DNA typing to identify the HLA alleles controlling low or high immune responses to SCW. HLA-DR2-DQA10102-DQB10602(DQw6)-Dw2 haplotype or HLA-DR2-DQA10103-DQB10601(DQw6)-DW12 haplotype was increased in frequency in the low responders and the frequency of HLA-DR4-DRw53-DQA10301-DQB10401(DQw4)-Dw15 haplotype or HLA-DR9-DRw53-DQA10301-DQB10303(DQw3)-Dw23 haplotype was increased in the high responders to SCW. Homozygotes of either DQA10102 or DQA10103 exhibited a low responsiveness to SCW and those of DQA10301 were high responders. The heterozygotes of DQA10102 or 0103 and DQA10301 showed a low response to SCW, thereby confirming that the HLA-linked gene controls the low response to SCW, as a dominant trait. Using mouse L cell transfectants expressing a single class II molecule as the APC, we found that DQw6(DQA10103 DQB10601) from the low responder haplotype (DR2-DQA10103-DQB10601(DQw6)-Dw12) activated SCW-specific T cell lines whereas DQw4(DQA10301 DQB10401) from the high responder haplotype (DR4-DRw53-DQA10301-DQB1*0401(DQw4)-Dw15) did not activate T cell lines specific to SCW. However, DR4 and DR2 presented SCW to CD4+ T cells in both the high and low responders to SCW, hence the DR molecule even from the low responder haplotype functions as an restriction molecule in the low responders. Putative mechanisms linked to the association between the existence of DQ-restricted CD4+ T cells specific to SCW, and low responsiveness to SCW are discussed.
