通过竞争性吸附研究皮质醇的蛋白质结合：应用于16种真兽亚纲哺乳动物血清对皮质醇的结合

Protein binding of cortisol by means of competitive adsorption: application to cortisol binding by serum of sixteen eutherian mammals.

Fenske M

Department of Animal Physiology, University of Bayreuth, FRG.

Comp Biochem Physiol A Comp Physiol. 1991;98(1):61-6. doi: 10.1016/0300-9629(91)90578-z.

Abstract

Binding of 3H-cortisol by serum proteins by means of competitive adsorption was relatively high by serum of the gerbil, human, rabbit, sheep, tree shrew, hamster, rhesus monkey and horse. 2. A somewhat lower binding was observed by serum proteins of the baboon, cattle, dog, rat and cat. 3. Serum taken from either the mouse, guinea pig or pig gave very flat binding curves, specific binding not exceeding 5% of added 3H-cortisol. 4. It is concluded that the measurement of protein-binding of 3H-cortisol by means of competitive adsorption is a reliable method for serum of most eutherian species but is unsuited if serum of the mouse, guinea pig or pig is used.

摘要

通过竞争性吸附，沙鼠、人类、兔子、绵羊、树鼩、仓鼠、恒河猴和马的血清对3H-皮质醇的蛋白结合率相对较高。2. 狒狒、牛、狗、大鼠和猫的血清蛋白结合率略低。3. 从小鼠、豚鼠或猪采集的血清给出的结合曲线非常平缓，特异性结合不超过添加的3H-皮质醇的5%。4. 得出结论，通过竞争性吸附测定3H-皮质醇的蛋白结合率，对于大多数真兽类物种的血清是一种可靠的方法，但如果使用小鼠、豚鼠或猪的血清则不适用。