Shan Jing, Wang Lu, Li Zhuo, Liu Ying, Gao Ji-rong, Pang Yan-lei, Li Jun-hong, Pang Fu-min, Guo Xin-hui, Chi Hong-Zhi, Zhang Chang-geng, Li Hui
Institute of Hepatitis Research, Affiliated You'an Hospital, Capital University of Medical Sciences, Beijing 100096, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2006 Apr;28(2):148-53.
To determine whether -Taq I T/C and -Fok I C/T polymorphisms of vitamin D receptor (VDR) gene are associated with the familial aggregation of hepatitis B virus (HBV) infection.
Based on a population-based case-control family design, 288 family members from 27 case families and 230 family members from 27 control families were recruited. VDR gene polymorphisms were analyzed. VDR-Taq I T/C and VDR-Fok I C/T polymorphisms were examined by polymerase chain reaction-restriction fragment length polymorphism.
The frequency of VDR-Taq I TT genotype in the case families was significantly higher than that in the control families (P < 0.05) , however, the frequency of VDR-Fok I CC genotype in the case families was significantly higher than that in the control families (P < 0.05). The frequency of family members carriying Taq I T-Fok I C haplotype in the case families was significantly higher than that in the control families (OR = 1.67, P < 0.05), however, the frequency of family members carrying Taq I C-Fok I T haplotype in the case families was significantly lower than that in the control families (OR = 0. 24, P < 0.05). The similar results were found in the familial biological kinship relatives with any HBV-infected makers.
VDR-Taq I and -Fok I gene polymorphisms are likely to play a substantial role in HBsAg familial aggregation.
确定维生素D受体（VDR）基因的-Taq I T/C和-Fok I C/T多态性是否与乙型肝炎病毒（HBV）感染的家族聚集性相关。
基于以人群为基础的病例对照家系设计，招募了来自27个病例家庭的288名家庭成员和来自27个对照家庭的230名家庭成员。分析VDR基因多态性。采用聚合酶链反应-限制性片段长度多态性方法检测VDR-Taq I T/C和VDR-Fok I C/T多态性。
病例家庭中VDR-Taq I TT基因型的频率显著高于对照家庭（P < 0.05），然而，病例家庭中VDR-Fok I CC基因型的频率显著高于对照家庭（P < 0.05）。病例家庭中携带Taq I T-Fok I C单倍型的家庭成员频率显著高于对照家庭（OR = 1.67，P < 0.05），然而，病例家庭中携带Taq I C-Fok I T单倍型的家庭成员频率显著低于对照家庭（OR = 0.24，P < 0.05）。在有任何HBV感染标志物的家族生物学亲属中也发现了类似结果。
VDR-Taq I和-Fok I基因多态性可能在HBsAg家族聚集中起重要作用。
