Wang Shi-jie, Guo Xiong, Ren Feng-ling, Zhang Yin-gang, Zhang Zeng-tie, Zhang Fu-jun, Geng Dong
Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Xi'an 710061, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2006 Apr;28(2):267-70.
To investigate chondrocyte apoptosis and expression of Fas and inducible nitric oxide synthase (iNOS) in articular cartilage in the pathogenesis of Kashin-beck disease (KBD) and primary osteoarthritis (OA).
The collected samples of articular cartilage were divided into three groups: normal control (15 cases), KBD adults (15 cases) and OA (15 cases). Chondrocyte apoptosis was detected by terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling method, and Fas and iNOS in articular cartilage were stained by immunohistochemistry.
The positive percentages of chondrocyte apoptosis stained in articular cartilage of KBD and OA were significantly higher than that of the control (P < 0.01), and the positive percentage of chondrocytes apoptosis in the eroded areas of articular cartilage were significantly higher than in the non-eroded areas in articular cartilage of the same patient with KBD and OA (P < 0.05). There was no significant difference in positive percentage of chondrocytes apoptosis between KBD and OA. The positive percentages of Fas and iNOS in chondrocytes were significantly higher in KBD and OA than in control (P < 0.01). Significant differences in Fas and iNOS expression between the eroded areas and non-eroded areas were seen in articular cartilage of patients with KBD and OA (P < 0.05), but such difference did not exist between KBD and OA.
Cell apoptosis seems to be associated with the pathogenesis of both KBD and OA. Fas and iNOS might mediate chondrocyte apoptosis.
探讨大骨节病（KBD）和原发性骨关节炎（OA）发病机制中关节软骨细胞凋亡及Fas和诱导型一氧化氮合酶（iNOS）的表达。
收集的关节软骨样本分为三组：正常对照组（15例）、KBD成人组（15例）和OA组（15例）。采用末端脱氧核苷酸转移酶生物素-dUTP缺口末端标记法检测软骨细胞凋亡，免疫组织化学法检测关节软骨中Fas和iNOS。
KBD和OA关节软骨中软骨细胞凋亡染色阳性率显著高于对照组（P<0.01），同一KBD和OA患者关节软骨侵蚀区软骨细胞凋亡阳性率显著高于非侵蚀区（P<0.05）。KBD和OA软骨细胞凋亡阳性率差异无统计学意义。KBD和OA软骨细胞中Fas和iNOS阳性率显著高于对照组（P<0.01）。KBD和OA患者关节软骨侵蚀区与非侵蚀区Fas和iNOS表达差异有统计学意义（P<0.05），但KBD与OA之间无差异。
细胞凋亡似乎与KBD和OA的发病机制有关。Fas和iNOS可能介导软骨细胞凋亡。
