Ringwald Jürgen, Haager Benedikt, Krex Daniel, Zimmermann Robert, Strasser Erwin, Antoon Marc, De Schrijver Eddy, Eckstein Reinhold
Department for Transfusion Medicine and Haemostaseology, University Hospital of Erlangen, Erlangen, Germany.
Transfusion. 2006 Jun;46(6):942-8. doi: 10.1111/j.1537-2995.2006.00826.x.
The quality of platelets (PLTs) stored in PLT additive solution (PAS) is dependent on the type and proportion of the used PAS. No data are available as to whether a different hold time before the addition of PAS to hyperconcentrated PLT suspensions has an impact on PLT quality. The in vitro quality between single-donor PLT concentrates was compared with two different hold times with two PASs.
On two occasions, 6x10(11) PLTs in 150 mL of plasma were collected from 20 blood donors. The units were split into two equal parts, and 140 mL of PAS-II or PAS-IIIM (randomized sequence) was added after 2 or 8 hours resulting in a PAS proportion of 65 percent. On Days 1, 5, and 7, glucose and lactate concentration, pH value, PLTs' P-selectin expression, response to hypotonic shock, and release of transforming growth factor-beta1 were determined.
On all days, the lactate concentrations were higher and pH values were lower in units with an 8-hour hold time, whereas the results of in vitro tests relating to the in vivo viability and activation of PLTs were similar for both groups. PAS-IIIM-stored PLTs showed a lower glycolytic activity and better results in all performed in vitro tests than PAS-II-stored PLTs.
Although the metabolism of glucose was enhanced during hold time, the differences between both hold time groups are not meaningful from a biological viewpoint. Therefore, an 8-hour hold time is feasible. PLT storage in PAS-IIIM results in a PLT in vitro quality superior to that of PLTs stored in PAS-II.
血小板添加剂溶液（PAS）中储存的血小板（PLT）质量取决于所用PAS的类型和比例。关于在高浓缩PLT悬浮液中添加PAS之前不同的保存时间是否会对PLT质量产生影响，目前尚无相关数据。比较了单采血小板浓缩物在两种不同保存时间下与两种PAS的体外质量。
在两个不同时间点，从20名献血者中采集150 mL血浆中的6×10¹¹个PLT。将这些单位分为两等份，在2小时或8小时后加入140 mL的PAS-II或PAS-IIIM（随机顺序），使PAS比例达到65%。在第1天、第5天和第7天，测定葡萄糖和乳酸浓度、pH值、PLT的P-选择素表达、对低渗休克的反应以及转化生长因子-β1的释放。
在所有天数中，保存8小时的单位中乳酸浓度较高，pH值较低，而两组关于PLT体内活力和活化的体外测试结果相似。与PAS-II储存的PLT相比，PAS-IIIM储存的PLT显示出较低的糖酵解活性，并且在所有进行的体外测试中结果更好。
尽管在保存期间葡萄糖代谢增强，但从生物学角度来看，两个保存时间组之间的差异并无意义。因此，8小时的保存时间是可行的。用PAS-IIIM储存PLT可使PLT的体外质量优于用PAS-II储存者。
