Santana Julie M, Dumont Larry J
R&D, Gambro BCT, Inc., Lakewood, Colorado 80215, USA.
Transfusion. 2006 Jun;46(6):966-72. doi: 10.1111/j.1537-2995.2006.00829.x.
Conventional automated cell counters cannot accurately count residual red blood cells (rRBCs) that are often present in various blood products. A two-color flow cytometric method (FC) was validated for detecting and enumerating rRBCs in platelets (PLTs) and mononuclear cell (MNC) products.
PLT and MNC products for PLTs (CD61-fluorescein isothiocyanate) and rRBC (anti-glycophorin A-phycoerythrin) were double stained, and data were acquired with a flow cytometer. Assay linearity, accuracy, and precision were assessed with a standard-dilution series of rRBCs. This assay was used to determine the rRBCs of apheresis PLTs collected with Trima Accel (Gambro BCT) and MNC products collected with the COBE Spectra (Gambro BCT).
The linear range of this assay in PLT and MNC products is 10 to 2000 RBCs per microL (R2=0.994). FC had a mean intraassay coefficient of variation of 11.8 percent at 34 RBCs per microL. A standard clinical hematology analyzer overestimated rRBCs in MNC products by 1.59x10(5)+/-0.7x10(5) RBCs per microL. Apheresis PLTs had a median of 17.4 RBCs per microL, with 99.0 percent containing fewer than 90.0 RBCs per microL.
This method for determining rRBCs in blood products is accurate and repeatable with a lower limit of detection adequate to assess currently available blood products. FC should be considered for determining rRBCs in MNC products.
传统的自动细胞计数器无法准确计数各种血液制品中常出现的残余红细胞（rRBC）。一种双色流式细胞术方法（FC）已被验证可用于检测和计数血小板（PLT）及单核细胞（MNC）制品中的rRBC。
对用于检测PLT（CD61 - 异硫氰酸荧光素）和rRBC（抗血型糖蛋白A - 藻红蛋白）的PLT和MNC制品进行双重染色，并用流式细胞仪获取数据。通过rRBC的标准稀释系列评估测定的线性、准确性和精密度。该测定法用于确定用Trima Accel（甘布罗BCT公司）采集的单采血小板的rRBC以及用COBE Spectra（甘布罗BCT公司）采集的MNC制品的rRBC。
该测定法在PLT和MNC制品中的线性范围为每微升10至2000个红细胞（R2 = 0.994）。FC在每微升34个红细胞时的平均批内变异系数为11.8%。一台标准临床血液学分析仪将MNC制品中的rRBC高估了每微升1.59×10(5)±0.7×10(5)个红细胞。单采血小板的中位数为每微升17.4个红细胞，99.0%的样本每微升含有的红细胞少于90.0个。
这种测定血液制品中rRBC的方法准确且可重复，检测下限足以评估目前可用的血液制品。在确定MNC制品中的rRBC时应考虑使用FC。
