[Cloning and analysis of gene encoding wheat translation initiation factor, eIF5A].

Eukaryotic translation initiation factor 5A (eIF5A) is involved in cell proliferation, senescence, development and environmental response. In this paper, DNA and cDNA of Chinese Spring was amplified by PCR with special primers, two special fragments of 1 679 bp and 1 910 bp were obtained with DNA as template and one special fragment of 636 bp was yielded with cDNA as template. Which were named eIF5a1 (GenBank number: DQ167202), eIF5a2 (Genbank number: DQ167201) and eIF5a3, respectively. The complete sequence (768 bp) of eIF5a3 (Genbank number: DQ167203) was obtained using GeneRacer kit. Nucleotide sequence analysis shows that eIF5a1 and eIF5a2 share identical transcriptions of 636bp which have only 6 nucleotides difference. Putative amino acid sequences analysis revealed that there was only one or two amino acid difference in eIF5a1, eIF5a2 and eIF5a3. Phylogenic analysis indicated that the eIF5A of wheat were relation to corn, rice, tomato and potato closely. In additional, eIF5a2 is located on chromosome 2B using Langdon D-genomic disomic substitution lines and the expression of eIF5A was also investigated by Semi-quantitative PCR.