Zhou Jian-Ping, Yang Zu-Jun, Feng Juan, Chi Shi-Hua, Liu Cheng, Ren Zheng-Long
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
Yi Chuan. 2006 May;28(5):571-7.
Eukaryotic translation initiation factor 5A (eIF5A) is involved in cell proliferation, senescence, development and environmental response. In this paper, DNA and cDNA of Chinese Spring was amplified by PCR with special primers, two special fragments of 1 679 bp and 1 910 bp were obtained with DNA as template and one special fragment of 636 bp was yielded with cDNA as template. Which were named eIF5a1 (GenBank number: DQ167202), eIF5a2 (Genbank number: DQ167201) and eIF5a3, respectively. The complete sequence (768 bp) of eIF5a3 (Genbank number: DQ167203) was obtained using GeneRacer kit. Nucleotide sequence analysis shows that eIF5a1 and eIF5a2 share identical transcriptions of 636bp which have only 6 nucleotides difference. Putative amino acid sequences analysis revealed that there was only one or two amino acid difference in eIF5a1, eIF5a2 and eIF5a3. Phylogenic analysis indicated that the eIF5A of wheat were relation to corn, rice, tomato and potato closely. In additional, eIF5a2 is located on chromosome 2B using Langdon D-genomic disomic substitution lines and the expression of eIF5A was also investigated by Semi-quantitative PCR.
真核生物翻译起始因子5A（eIF5A）参与细胞增殖、衰老、发育及环境应答。本文以中国春小麦的DNA和cDNA为模板，用特异引物通过PCR进行扩增，以DNA为模板获得了1679bp和1910bp的两个特异片段，以cDNA为模板获得了636bp的一个特异片段，分别命名为eIF5a1（GenBank登录号：DQ167202）、eIF5a2（Genbank登录号：DQ167201）和eIF5a3。使用GeneRacer试剂盒获得了eIF5a3（Genbank登录号：DQ167203）的完整序列（768bp）。核苷酸序列分析表明，eIF5a1和eIF5a2具有636bp的相同转录本，仅相差6个核苷酸。推测的氨基酸序列分析显示，eIF5a1、eIF5a2和eIF5a3之间只有一两个氨基酸差异。系统发育分析表明，小麦的eIF5A与玉米、水稻、番茄和马铃薯关系密切。此外，利用Langdon D基因组二体代换系将eIF5a2定位到2B染色体上，并通过半定量PCR对eIF5A的表达进行了研究。
