转运核糖核酸的修饰核苷。II. 2'-O-甲基胞苷酰（3'-5'）胞苷的合成。
The modified nucleosides of tRNAs. II. Synthesis of 2'-O-methylcytidylyl (3'-5') cytidine.
作者信息
Markiewicz W T, Wiewiórowski M
出版信息
Nucleic Acids Res. 1975 Jun;2(6):951-60. doi: 10.1093/nar/2.6.951.
Abstract
The synthesis of 2'-O-methylcytidylyl (3'-5')cytidine by the triester method using as protecting groups, 2,2,2-trichloroethyl for phosphate hydroxyl group, p-chlorophenyoxyacetyl for 5-hydroxyl group, methoxymethylidene for 2',3'-cis-diol system, and benzoyl for the exo-amino group of cytidine is presented. The obtained product was characterised by UV, electrophoresis, chromatography and an enzymatic digestion.
摘要
介绍了使用2,2,2-三氯乙基作为磷酸羟基的保护基团、对氯苯氧基乙酰基作为5-羟基的保护基团、甲氧基亚甲基作为2',3'-顺式二醇系统的保护基团以及苯甲酰基作为胞苷的外氨基保护基团，通过三酯法合成2'-O-甲基胞苷酰基(3'-5')胞苷。所得产物通过紫外光谱、电泳、色谱和酶消化进行表征。
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2
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