Vandeplassche G, Lu H R, Wouters L, Flameng W, Borgers M
Janssen Research Foundation, Belgium.
Basic Res Cardiol. 1991 Jan-Feb;86(1):21-31. doi: 10.1007/BF02193868.
The effect of pretreatment with selective beta 1-adrenoceptor blockers (dl-nebivolol or atenolol) on myocardial mechanical activity, mitochondrial function, morphology, and calcium cytochemistry was studied during normothermic ischemic arrest and reperfusion of isolated working rabbit hearts. The hearts subjected to 25 min of ischemia followed by 30 min of post-ischemic reperfusion showed typical signs of severe myocardial ischemic damage. The ultrastructural changes showed a good relation with the changes in mechanical activity and mitochondrial function. To determine whether these changes could be prevented or reduced by beta 1-adrenoceptor blockade, dl-nebivolol or atenolol (0.62 mg/liter) was added to the perfusate 30 min before the induction of ischemia. The results showed that dl-nebivolol exerted a protective effect on recovery of mechanical activity, on mitochondrial function during reperfusion as well as on the ultrastructure as examined at the end of the reperfusion period. On the other hand, atenolol failed to protect the myocardium against ischemia-reperfusion damage in the isolated working rabbit heart.
在离体工作兔心的常温缺血性停搏和再灌注期间，研究了用选择性β1肾上腺素能受体阻滞剂（右旋奈必洛尔或阿替洛尔）预处理对心肌机械活动、线粒体功能、形态以及钙细胞化学的影响。经历25分钟缺血后再进行30分钟缺血后再灌注的心脏表现出严重心肌缺血损伤的典型征象。超微结构变化与机械活动和线粒体功能的变化密切相关。为了确定这些变化是否可以通过β1肾上腺素能受体阻滞来预防或减轻，在缺血诱导前30分钟将右旋奈必洛尔或阿替洛尔（0.62毫克/升）加入灌注液中。结果表明，右旋奈必洛尔对机械活动的恢复、再灌注期间的线粒体功能以及再灌注期末检查的超微结构均有保护作用。另一方面，阿替洛尔未能保护离体工作兔心的心肌免受缺血-再灌注损伤。
