Zhao Xin-Cheng, Yan Yun-Hua, Wang Chen-Zhu
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, The Chinese Academy of Sciences, Beijing 100080, China.
J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2006 Oct;192(10):1037-47. doi: 10.1007/s00359-006-0141-6. Epub 2006 May 31.
Two sibling species, Helicoverpa assulta and Helicoverpa armigera both use (Z)-9-hexadecenal and (Z)-11-hexadecenal as their sex pheromone components but in almost reversed ratios, 93:7 and 3:97, respectively. H. assulta and H. armigera males performed upwind flight in response to the H. assulta sex pheromone blend (93:7). H. armigera responded strongly to the H. armigera blend (3:97), whereas H. assulta males remained inactive upon exposure to this blend. Both species gave clear dose-dependent electrophysiological responses to (Z)-11-hexadecenal. However, (Z)-9-hexadecenal evoked strong dose-dependent electrophysiological responses in H. assulta males but not in H. armigera. The two male F1 hybrids exhibited similar behavioral responses to two sex pheromone blends and electrophysiological responses to two pheromone components as H. armigera males. This indicated that H. armigera genes appear dominant in determining the behavioral response and electrophysiological responses. Behavioral and electrophysiological responses of backcrosses of male F1 hybrids (H. armigera female x H. assulta male) with female H. assulta and H. armigera were close to that of H. assulta and H. armigera, respectively. However, backcrosses of female F1 hybrids (H. assulta female x H. armigera male) with male H. assulta and H. armigera showed reduced behavioral responses but normal electrophysiological responses compared to males of the respective parental line.
棉铃虫和烟青虫这两个近缘种都利用（Z）-9-十六碳烯醛和（Z）-11-十六碳烯醛作为性信息素成分，但比例几乎相反，分别为93:7和3:97。棉铃虫和烟青虫的雄虫对棉铃虫性信息素混合物（93:7）做出逆风飞行反应。烟青虫对烟青虫混合物（3:97）反应强烈，而棉铃虫雄虫接触该混合物后仍不活跃。两种昆虫对（Z）-11-十六碳烯醛都有明显的剂量依赖性电生理反应。然而，（Z）-9-十六碳烯醛在棉铃虫雄虫中引起强烈的剂量依赖性电生理反应，而在烟青虫中则没有。两个雄性F1杂种对两种性信息素混合物的行为反应以及对两种信息素成分的电生理反应与烟青虫雄虫相似。这表明烟青虫基因在决定性行为反应和电生理反应方面似乎占主导地位。雄性F1杂种（烟青虫雌虫×棉铃虫雄虫）与棉铃虫雌虫和烟青虫雌虫回交的行为和电生理反应分别接近棉铃虫和烟青虫。然而，雌性F1杂种（棉铃虫雌虫×烟青虫雄虫）与棉铃虫雄虫和烟青虫雄虫回交时，与各自亲本品系的雄虫相比，行为反应减弱，但电生理反应正常。
