Mei Shuang-Shuang, Li Yun, Lu Qiu-He, Xiang Hua
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing L00080, China.
Wei Sheng Wu Xue Bao. 2006 Apr;46(2):318-22.
Halocin C8 (HalC8), produced by a halophilic archaeon strain AS7092, is a gene-coded peptide microhalocin and has a wide inhibitory spectrum against the members of haloarchaea. To investigate the mechanisms of the gene expression regulation, the peptide processing and transportation of this halocin, a 9.3kb DNA gene cluster containing the halocin C8 encoding gene (proC8) and other possible involved genes was cloned, by screening of a genomic library of AS7092 as well as anchoring PCR technique. Sequence analysis indicated that it contained at least six open reading frames, including halU, halR, proC8, halT1, halT2 and halT3. The gene halU encodes a membrane-spanning protein HalU, but its function is unknown. The gene halR encodes a putative regulator protein HalR, its function was deduced to regulate the transcription of proC8 gene, which encodes the precursor for halocin C8. The gene halT1, halT2 and halT3 likely encode the transporters HalT1, HalT2 and HalT3, the functions of which were speculated to transport the halocin C8 out of the cellular membrane. This is the first report of gene cluster cloning for any halocin.
嗜盐古菌菌株AS7092产生的卤霉素C8（HalC8）是一种基因编码的肽类微卤霉素，对嗜盐古菌成员具有广泛的抑制谱。为了研究该卤霉素的基因表达调控、肽加工和转运机制，通过筛选AS7092的基因组文库以及锚定PCR技术，克隆了一个包含卤霉素C8编码基因（proC8）和其他可能相关基因的9.3kb DNA基因簇。序列分析表明，它至少包含六个开放阅读框，包括halU、halR、proC8、halT1、halT2和halT3。基因halU编码一种跨膜蛋白HalU，但其功能未知。基因halR编码一种假定的调节蛋白HalR，推测其功能是调节proC8基因的转录，该基因编码卤霉素C8的前体。基因halT1、halT2和halT3可能编码转运蛋白HalT1、HalT2和HalT3，推测其功能是将卤霉素C8转运出细胞膜。这是关于任何卤霉素基因簇克隆的首次报道。
