Itano E N, Ono M A, Sumigawa M, Longo I M, Moura N C, Mendes N F
Departamento de Patologia Geral, Universidade Estadual de Londrina, Paraná, Brazil.
J Clin Lab Anal. 1991;5(2):114-20. doi: 10.1002/jcla.1860050208.
Using a specific serum anti-soluble T lymphocytes receptor for sheep erythrocytes (E) and SDS-PAGE, we detected radioactive bands of molecular weight 58,000 in immunoprecipitates of supernatant of heated human lymphocytes (SHL), in the supernatant of PHA stimulated lymphocyte cultures (SLC), normal human serum (NHS), and serum from cancer and uremia patients, labelled with 131I. By Sephadex G-200 chromatography, in addition to this fraction, we detected molecules of molecular weight higher than 150,000 which interact with the anti-soluble receptor serum (anti-RS), in serum from cancer and uremia patients. These molecules were detected in NHS or SHL after concentration or by prolonged exposure of SDS-PAGE with some labelled and immunoprecipitated SHL samples. The soluble receptors of molecular weights 58,000 (RS1) and more than 150,000 (RS2) were fully identical when analyzed by immunodiffusion with anti-RS serum. When submitted to immunoelectrophoresis, RS1 showed electrophoretic migration similar to that of albumin, while RS2 showed a pattern close to that of alpha 2-globulin. However, RS2 did not show antigenic relationship with IgM and was not an immune complex with IgG. Even though the presence of RS in human saliva has not yet been reported, molecules that interact with anti-RS serum have been detected in human saliva and are fully identical to molecules found in supernatant of heated human T lymphocytes and NHS. The RS molecules present in human saliva have a molecular weight and electrophoretic migration similar to those of RS1 from SLC and from human serum and have no antigenic relationship with human albumin.
我们使用一种针对绵羊红细胞（E）的特异性血清抗可溶性T淋巴细胞受体，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE），在经131I标记的加热人淋巴细胞上清液（SHL）、PHA刺激的淋巴细胞培养上清液（SLC）、正常人血清（NHS）以及癌症和尿毒症患者的血清的免疫沉淀物中，检测到了分子量为58,000的放射性条带。通过葡聚糖G-200柱层析，除了该部分外，我们在癌症和尿毒症患者的血清中还检测到了分子量高于150,000且与抗可溶性受体血清（抗RS）相互作用的分子。在对NHS或SHL进行浓缩后，或者通过对一些标记并免疫沉淀的SHL样品进行长时间的SDS-PAGE曝光，也检测到了这些分子。当用抗RS血清通过免疫扩散分析时，分子量为58,000（RS1）和超过150,000（RS2）的可溶性受体完全相同。当进行免疫电泳时，RS1显示出与白蛋白相似的电泳迁移，而RS2显示出与α2球蛋白相近的图谱。然而，RS2与IgM没有抗原关系，也不是与IgG形成的免疫复合物。尽管尚未有关于人唾液中存在RS的报道，但在人唾液中已检测到与抗RS血清相互作用的分子，且这些分子与在加热人T淋巴细胞上清液和NHS中发现的分子完全相同。人唾液中存在的RS分子的分子量和电泳迁移与来自SLC和人血清的RS1相似，并且与人白蛋白没有抗原关系。
