An Ya-chen, Feng Fu-min, Yuan Ju-xiang, Ji Chun-mei, Wang Yu-hua, Guo Mei, Deng Xiao-juan, Gao Bao-xia, Wang Dong, Liu Qian
Epidemiological Department of North China Coal Medical College, Tangshan 063000, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2006 Jan;27(1):37-40.
To investigate the association between natural-resistance-associated macrophage protein 1 (NRAMP1) gene polymorphisms and susceptibility to pulmonary tuberculosis (TB) in Han nationality from Northern part of China.
A 1:1 matched case-control study was adopted. Polymerase chain reaction and restriction fragment length polymorphism technique were used to type the two NRAMP1 polymorphisms: INT4 and 3'UTR. Information on environmental-related risk factors and pathological changes of tuberculosis was collected using a pre-tested standard questionnaire. Univariate and multivariate conditional logistic analyses were conducted using SPSS for window software.
A sample consisting 124 pairs of cases and controls was studied. Univariate analysis demonstrated that the 3'UTR TGTG+/del genotype occurred more frequently in the cases than in the controls, with crude OR (95% CI) being 2.923 (1.557 - 5.487). No significant association was observed between TB and INT4 polymorphism. In multivariate analysis, associations of TB and 3'UTR TGTG+/del genotype remained, after adjusting for scar of bacillus Calmette-Guérin vaccine, marriage status, body mass index and exposure history. Adjusted OR (95% CI) was 2.955 (1.369 - 6.381). Again, no significant association between INT4 polymorphism and TB was found. Among different INT4 genotypes, the pathological characters of pulmonary tuberculosis were also found different (chi(2) = 9.634, P < 0.05).
Polymorphism of 3'UTR locus in NRAMP1 gene might affect their susceptibility to TB in Han nationality living in the northern part of China, and polymorphism of INT4 might affect the pathological characters of tuberculosis.
探讨自然抗性相关巨噬细胞蛋白1（NRAMP1）基因多态性与中国北方汉族人群肺结核易感性的关系。
采用1:1匹配的病例对照研究。运用聚合酶链反应和限制性片段长度多态性技术对NRAMP1基因的两个多态性位点：INT4和3'UTR进行分型。使用预先测试的标准问卷收集与环境相关的危险因素和结核病病理变化的信息。使用SPSS for Windows软件进行单因素和多因素条件逻辑分析。
对124对病例和对照组成的样本进行了研究。单因素分析表明，病例组中3'UTR TGTG+/del基因型的出现频率高于对照组，粗OR（95%CI）为2.923（1.557 - 5.487）。未观察到结核病与INT4多态性之间存在显著关联。在多因素分析中，调整卡介苗疤痕、婚姻状况、体重指数和接触史后，结核病与3'UTR TGTG+/del基因型之间的关联仍然存在。调整后的OR（95%CI）为2.955（1.369 - 6.381）。同样，未发现INT4多态性与结核病之间存在显著关联。在不同的INT4基因型中，还发现肺结核的病理特征存在差异（χ² = 9.634，P < 0.05）。
NRAMP1基因3'UTR位点的多态性可能影响中国北方汉族人群对结核病的易感性，而INT4多态性可能影响结核病的病理特征。
