Wang Fang, Zhao Lin-qing, Deng Jie, Zhu Ru-nan, Qian Yuan
Laboratory of virology, Beijing Municipal Laboratory of Infection and Immunity, Capital Institute of Pediatrics, Beijing 100020, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2006 Jan;27(1):44-6.
To understand the relationship of parainfluenza virus (PIV) and acute respiratory infections in infants and young children in Beijing, occurred in recent years.
3141 throat swab/nasopharyngeal aspirate specimens were collected from infants and young children with acute respiratory tract infections in Beijing from Jan 2001 to Dec 2003. All of these 3141 specimens were inoculated into MDCK cells for influenza virus and PIV isolation, since PIV had been isolated in MDCK cells in this laboratory from preliminary studies. Out of 3141 specimens, 702 were inoculated into MDCK as well as Vero cells to compare the sensitivity on virus isolation of these cell lines by micro plate method. Growth of PIV in cell culture were identified by haemoagglutination test and indirect immunofluorescent assay.
The PIV positive cases in Vero cells were also positive in MDCK cells, indicating that the sensitivity for PIV isolation in MDCK was equal to Vero cells. Out of these 3141 specimens, 94 (3.0%) were PIV positive, including 35 (35/1191, 2.9%) of PIV1, 11 (11/1191, 0.9%) of PIV3 in upper respiratory tract infections; 15 (15/1634, 0.9%) of PIV1, 24 (24/1634, 1.5%) of PIV3 in lower respiratory tract infections; 3 (3/207, 1.4%) of PIV in asthma; 1 (1/38) of PIV in patients with fever; 5 (5/71) of PIV in others. Data indicated that among upper respiratory tract infections caused by PIV, PIV1 was more commonly seen than PIV3.
MDCK cells could be used for PIV isolation from clinical samples while PIV was one of the important pathogenic viruses causing acute respiratory tract infections in infants and young children in Beijing for the recente years.
了解近年来北京地区婴幼儿副流感病毒（PIV）与急性呼吸道感染的关系。
收集2001年1月至2003年12月期间北京地区患有急性呼吸道感染的婴幼儿的3141份咽拭子/鼻咽抽吸物标本。由于本实验室前期研究已证实PIV可在MDCK细胞中分离，因此将所有这3141份标本接种于MDCK细胞进行流感病毒和PIV分离。在3141份标本中，702份同时接种于MDCK细胞和Vero细胞，采用微量板法比较这两种细胞系对病毒分离的敏感性。通过血凝试验和间接免疫荧光法鉴定细胞培养中PIV的生长情况。
Vero细胞中PIV阳性的病例在MDCK细胞中也呈阳性，表明MDCK细胞对PIV分离的敏感性与Vero细胞相当。在这3141份标本中，94份（3.0%）PIV阳性，其中上呼吸道感染中PIV1为35份（35/1191，2.9%），PIV3为11份（11/1191，0.9%）；下呼吸道感染中PIV1为15份（15/1634，0.9%），PIV3为24份（24/1634，1.5%）；哮喘患者中PIV为3份（3/207，1.4%）；发热患者中PIV为1份（1/38）；其他患者中PIV为5份（5/71）。数据表明，在PIV引起的上呼吸道感染中，PIV1比PIV3更常见。
MDCK细胞可用于从临床标本中分离PIV，而PIV是近年来北京地区婴幼儿急性呼吸道感染的重要致病病毒之一。
