[细胞色素P450 1A1（CYP-1A1）、细胞色素P450 2D6（CYP2D6）基因多态性与慢性苯中毒风险]

[Genetic polymorphism of CYP-1A1, CYP2D6 and risks of chronic benzene poisoning].

作者信息

Gu Shou-yong, Zhang Zhong-bin, Cao Duo-zhi, Wan Jun-xiang, Gao Xiao-ling, Jin Xi-peng, Xia Zhao-lin

机构信息

Department of Occupational Health, School of Public Health, Fudan University, Shanghai 200032, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2006 May;24(5):266-9.

PMID:16737584

Abstract

OBJECTIVE

To explore the relationship between genetic polymorphisms of CYP-1A1 and CYP2D6 and risks of chronic benzene poisoning (BP).

METHODS

A case control study was conducted. 152 BP patients and 152 workers occupationally exposed to benzene without poisoning manifestations were involved. Polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) technology was used for detecting the single nucleotide polymorphisms (SNPs) of MspI in the non-coding region of CYP-1A1 gene and c.188, g.212 position in the first extron of CYP2D6 gene.

RESULTS

The individuals with CYP1A1 MspI T/T genotype had a 1.32 times (95% CI: 1.05 approximately 1.65, P = 0.02) increased risk of BP compared with those carrying T/C and C/C genotypes. In no-smoking population, there was a 1.56 times (95% CI: 1.15 approximately 2.12, P = 0.003) increased risk of BP for subjects carrying CYP1A1 MspIT/T genotype compared with those carrying T/C and C/C genotypes. The individuals carrying CYP2D6 c.188 C/C or C/T genotype had a 1.23 times (95% CI: 1.05 approximately 1.42, P = 0.01) increased risk compared with those carrying T/T genotypes. In no-smoking population, there was a 1.23 times (95% CI: 1.04 approximately 1.47, P = 0.01) increased risk of BP for subjects carrying CYP2D6 c.188 C/C or C/T genotypes compared with those carrying T/T genotype. The single nucleotide polymorphism of g.212 position in the first extron of CYP2D6 gene had not been validated.

CONCLUSION

The individuals with CYP2D6 c.188 C/C, CYP2D6 c.188 C/T and CYP1A1 MspIT/T genotypes tend to be more susceptible to benzene toxicity.

摘要

目的

探讨细胞色素P450 1A1（CYP-1A1）和细胞色素P450 2D6（CYP2D6）基因多态性与慢性苯中毒（BP）风险之间的关系。

方法

进行病例对照研究。纳入152例BP患者和152例无中毒表现的职业性接触苯的工人。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测CYP-1A1基因非编码区MspI的单核苷酸多态性（SNP）以及CYP2D6基因第一外显子中c.188、g.212位点的SNP。

结果

与携带CYP1A1 MspI T/C和C/C基因型的个体相比，CYP1A1 MspI T/T基因型个体发生BP的风险增加1.32倍（95%置信区间：1.05至1.65，P = 0.02）。在不吸烟人群中，与携带CYP1A1 MspI T/C和C/C基因型的个体相比，携带CYP1A1 MspI T/T基因型的个体发生BP的风险增加1.56倍（95%置信区间：1.15至2.12，P = 0.003）。与携带CYP2D6 T/T基因型的个体相比，携带CYP2D6 c.188 C/C或C/T基因型的个体发生BP的风险增加1.23倍（95%置信区间：1.05至1.42，P = 0.01）。在不吸烟人群中，与携带CYP2D6 T/T基因型的个体相比，携带CYP2D6 c.188 C/C或C/T基因型的个体发生BP的风险增加1.23倍（95%置信区间：1.04至1.47，P = 0.）。CYP2D6基因第一外显子中g.212位点的单核苷酸多态性未得到验证。