NG108-15神经母细胞瘤×胶质瘤杂交细胞中的磷酸肌醇与突触功能

Phosphoinositides and synaptic function in NG108-15 neuroblastoma x glioma hybrid cells.

Higashida H, Yokoyama S, Hoshi N, Myojo Y, Kawamura T, Ito Y, Hashii M, Sagara J, Furuya K

Department of Biophysics, Kanazawa University School of Medicine, Japan.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1991;98(1):129-37.

Abstract

The second-messengers system of bradykinin (BK) receptors was examined in NG108-15 neuroblastoma x glioma hybrid cells. 2. An application of BK induced an immediate outward (K+) current and acetylcholine (ACh) release, which are generated through inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ ions. 3. Application of phorbol dibutyrate (a protein kinase C activator) produced a voltage-dependent inward current and inhibited another K+ (M)-current. 4. A similar current response has been produced by ACh in NG108-15 cells transfected with rodent muscarinic ACh receptor I and III subtype genes. 5. These results suggest a dual and time-dependent role for these two intracellular messengers in the control of neuronal signalling by BK and ACh.

摘要

在NG108 - 15神经母细胞瘤×胶质瘤杂交细胞中检测了缓激肽（BK）受体的第二信使系统。2. 应用BK可诱导即刻外向（K +）电流和乙酰胆碱（ACh）释放，它们是通过依赖于肌醇1,4,5 - 三磷酸（InsP3）的Ca2 +离子产生的。3. 应用佛波二丁酸酯（一种蛋白激酶C激活剂）产生电压依赖性内向电流并抑制另一种K +（M）电流。4. 在转染了啮齿动物毒蕈碱型ACh受体I和III亚型基因的NG108 - 15细胞中，ACh也产生了类似的电流反应。5. 这些结果表明这两种细胞内信使在BK和ACh对神经元信号传导的控制中具有双重且时间依赖性的作用。